Phytochemical, Proximate and Anti-Microbial Screening of Methanol Extract of Populus grandidentata (big-tooth aspen) collected from Uvwie Local Government Area in Delta State, Nigeria

Abstract

Throughout antiquity till the modern age, medicinal plants have been used worldwide in traditional and ethnomedical medicine. Hence, the objective of this paper is to present the phytochemical, proximate and anti- microbial screening of methanol extract of Populus grandidentata (big-tooth aspen) collected from Uvwie Local Government Area in Delta State, Nigeria using. Proximate analysis, antimicrobial analysis, and phytochemical screening were performed on the obtained extract. The findings demonstrate the presence of saponin, flavonoids, alkaloids, glycosides, tannins, and steroids in the phytochemical screening of the investigated plant. The elemental analysis revealed the following percentages to be present: nitrogen (5.64%), carbon (37.46%), hydrogen (3.50%), and sulfur (0.68%). The proximate analysis resulted in low moisture content of 1.9%, low lipid content of 20.9%, and low ash content of 15.6%. Antimicrobial efficacy of Populus grandidentata leaf was examined using standard laboratory bacterial isolates. With a zone of inhibition ranging between 22 and 27 mm as compared with standard dugs, the isolates of bacteria and fungi include Methicillin Resistant Staphaureus, Vanomycin Resistant Enterococci, Staphylococcus, Escherichia coli, Helicobacter pyroli, Campylobacter jejuni, Proteus mirabilis, Candida albicans, Candida tropicalis, and Candida krusei. All of the microbes had minimum inhibitory concentrations of 25 ug/ml and minimum fungicidal and bactericidal concentrations of 50 ug/ml, respectively.

Keywords

• Populus grandidentata; Antimicrobial activity; proximate analysis; Ethnomedical medicine; Big-tooth aspen