Generation of precision-cut slice cultures of human placenta

Amal Fahmi; Melanie Brügger; Beatrice Zumkehr; Blandina I. Oliveira Esteves; David Baud; Marco P. Alves

STAR Protocols (Jun 2022)

Generation of precision-cut slice cultures of human placenta

Amal Fahmi,
Melanie Brügger,
Beatrice Zumkehr,
Blandina I. Oliveira Esteves,
David Baud,
Marco P. Alves

Affiliations

Amal Fahmi: Institute of Virology and Immunology, Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland; Corresponding author
Melanie Brügger: Institute of Virology and Immunology, Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Corresponding author
Beatrice Zumkehr: Institute of Virology and Immunology, Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland
Blandina I. Oliveira Esteves: Institute of Virology and Immunology, Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland
David Baud: Materno-Fetal and Obstetrics Research Unit, Department ''Femme-Mère-Enfant'', Lausanne University Hospital, Lausanne, Switzerland
Marco P. Alves: Institute of Virology and Immunology, Bern, Switzerland; Department of Infectious Diseases and Pathobiology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Corresponding author

Journal volume & issue: Vol. 3, no. 2
p. 101291

Abstract

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Summary: We present a protocol to generate an advanced ex vivo model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular composition comparable to the native tissue. We applied this system to study SARS-CoV-2 infection at the maternal-fetal interface. Moreover, this system can be used to investigate the basic functions of the human placenta in health and disease.For complete details on the use and execution of this protocol, please refer to Fahmi et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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