Frontiers in Immunology (Sep 2022)
Longitudinal profiles of plasma gelsolin, cytokines and antibody expression predict COVID-19 severity and hospitalization outcomes
- Meshach Asare-Werehene,
- Meshach Asare-Werehene,
- Meshach Asare-Werehene,
- Meshach Asare-Werehene,
- Michaeline McGuinty,
- Michaeline McGuinty,
- Michaeline McGuinty,
- Agatha Vranjkovic,
- Agatha Vranjkovic,
- Agatha Vranjkovic,
- Yannick Galipeau,
- Juthaporn Cowan,
- Juthaporn Cowan,
- Juthaporn Cowan,
- Juthaporn Cowan,
- Bill Cameron,
- Bill Cameron,
- Bill Cameron,
- Bill Cameron,
- Curtis L. Cooper,
- Curtis L. Cooper,
- Curtis L. Cooper,
- Marc-André Langlois,
- Marc-André Langlois,
- Angela M. Crawley,
- Angela M. Crawley,
- Angela M. Crawley,
- Angela M. Crawley,
- Angela M. Crawley,
- Angela M. Crawley,
- Benjamin K. Tsang,
- Benjamin K. Tsang,
- Benjamin K. Tsang,
- Benjamin K. Tsang
Affiliations
- Meshach Asare-Werehene
- Department of Obstetrics & Gynecology, University of Ottawa, Ottawa, ON, Canada
- Meshach Asare-Werehene
- Department of Cellular & Molecular Medicine, University of Ottawa, Ottawa, ON, Canada
- Meshach Asare-Werehene
- Centre for Infection, Immunity & Inflammation, University of Ottawa, Ottawa, ON, Canada
- Meshach Asare-Werehene
- Chronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada
- Michaeline McGuinty
- Department of Medicine, University of Ottawa, Ottawa, ON, Canada
- Michaeline McGuinty
- Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, Canada
- Michaeline McGuinty
- Clinical Epidemiology Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada
- Agatha Vranjkovic
- Chronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada
- Agatha Vranjkovic
- Department of Medicine, University of Ottawa, Ottawa, ON, Canada
- Agatha Vranjkovic
- Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, Canada
- Yannick Galipeau
- Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, Canada
- Juthaporn Cowan
- Centre for Infection, Immunity & Inflammation, University of Ottawa, Ottawa, ON, Canada
- Juthaporn Cowan
- Department of Medicine, University of Ottawa, Ottawa, ON, Canada
- Juthaporn Cowan
- Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, Canada
- Juthaporn Cowan
- Clinical Epidemiology Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada
- Bill Cameron
- Centre for Infection, Immunity & Inflammation, University of Ottawa, Ottawa, ON, Canada
- Bill Cameron
- Department of Medicine, University of Ottawa, Ottawa, ON, Canada
- Bill Cameron
- Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, Canada
- Bill Cameron
- Clinical Epidemiology Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada
- Curtis L. Cooper
- Department of Medicine, University of Ottawa, Ottawa, ON, Canada
- Curtis L. Cooper
- Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, Canada
- Curtis L. Cooper
- Clinical Epidemiology Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada
- Marc-André Langlois
- Centre for Infection, Immunity & Inflammation, University of Ottawa, Ottawa, ON, Canada
- Marc-André Langlois
- Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, Canada
- Angela M. Crawley
- Centre for Infection, Immunity & Inflammation, University of Ottawa, Ottawa, ON, Canada
- Angela M. Crawley
- Chronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada
- Angela M. Crawley
- Department of Medicine, University of Ottawa, Ottawa, ON, Canada
- Angela M. Crawley
- Department of Biochemistry, Microbiology & Immunology, University of Ottawa, Ottawa, ON, Canada
- Angela M. Crawley
- Department of Biology, Carleton University, Ottawa, ON, Canada
- Angela M. Crawley
- Coronavirus Variants Rapid Response Network–Biobank, Ottawa, ON, Canada
- Benjamin K. Tsang
- Department of Obstetrics & Gynecology, University of Ottawa, Ottawa, ON, Canada
- Benjamin K. Tsang
- Department of Cellular & Molecular Medicine, University of Ottawa, Ottawa, ON, Canada
- Benjamin K. Tsang
- Centre for Infection, Immunity & Inflammation, University of Ottawa, Ottawa, ON, Canada
- Benjamin K. Tsang
- Chronic Disease Program, Ottawa Hospital Research Institute, Ottawa, ON, Canada
- DOI
- https://doi.org/10.3389/fimmu.2022.1011084
- Journal volume & issue
-
Vol. 13
Abstract
BackgroundPrognostic markers for COVID-19 disease outcome are currently lacking. Plasma gelsolin (pGSN) is an actin-binding protein and an innate immune marker involved in disease pathogenesis and viral infections. Here, we demonstrate the utility of pGSN as a prognostic marker for COVID-19 disease outcome; a test performance that is significantly improved when combined with cytokines and antibodies compared to other conventional markers such as CRP and ferritin.MethodsBlood samples were longitudinally collected from hospitalized COVID-19 patients as well as COVID-19 negative controls and the levels of pGSN in μg/mL, cytokines and anti- SARS-CoV-2 spike protein antibodies assayed. Mean ± SEM values were correlated with clinical parameters to develop a prognostic platform.ResultspGSN levels were significantly reduced in COVID-19 patients compared to healthy individuals. Additionally, pGSN levels combined with plasma IL-6, IP-10 and M-CSF significantly distinguished COVID-19 patients from healthy individuals. While pGSN and anti-spike IgG titers together strongly predict COVID-19 severity and death, the combination of pGSN and IL-6 was a significant predictor of milder disease and favorable outcomes.ConclusionTaken together, these findings suggest that multi-parameter analysis of pGSN, cytokines and antibodies could predict COVID-19 hospitalization outcomes with greater certainty compared with conventional clinical laboratory markers such as CRP and ferritin. This research will inform and improve clinical management and health system interventions in response to SARS-CoV-2 infection.
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