Neuroprotective effects of nanogold-based Ayurveda medicine Suvarna Bhasma against rotenone-induced Parkinson's-like model

Abstract

Background: Neurodegenerative diseases have been one of the major concerns for human health. Genetic and environmental factors are believed to be responsible for neuronal diseases such as Parkinson's disease, Alzheimer's disease, and Huntington's disease. It is difficult to restore normal nervous function after neurodegeneration; hence, prevention could be the best strategy against these diseases. Ayurved medicines such as Suvarna Bhasma (SB) have enormous potential to treat these neurological diseases. Aim: The aim of this study is to examine the protective effect of SB against rotenone-induced Parkinson's-like model in zebrafish. Materials and methods: In this study, we induced Parkinson's-like disease model in zebrafish by inducing it with rotenone (7 μg/L). We examined the behavioural, proteomics and dopamine alterations of rotenone induced zebrafish of SB pre-treated group as compared to the control group. Results: The behavioural experiments showed that due to rotenone exposure, Parkinson's-like behavioural abnormality was induced in zebrafish. However, because of SB treatment, this behavioural abnormality was reduced. The proteomics study of zebrafish brains clearly showed that the SB-treated group was not significantly affected due to rotenone exposure. However, in the SB non-treated group, expression of nine proteins that are linked to Parkinson's disease (gene name: sncgb, ywhae1, ywhah, uchl1, ywhaba, psma6a, ywhabl, ywhaqb, and ywhabb) were differentially expressed after rotenone exposure. Finally, prevention of dopamine alteration in SB-treated fish brains confirmed the protective action of SB against rotenone-induced Parkinson's-like model in zebrafish. Conclusions: This study finds that Suvarna Bhasma has neuroprotective effects against Parkinson's-like disease model.

Keywords

• Parkinson's
• Suvarna bhasma
• Rotenone
• Dopamine
• Neuroprotection