Hypoglycemic Activity of Aqueous Extract of Latex from <i>Hancornia speciosa</i> Gomes: A Study in Zebrafish and In Silico

Rosana Tomazi; Ângela Costa Figueira; Adriana Maciel Ferreira; Diego Quaresma Ferreira; Gisele Custódio de Souza; Wandson Braamcamp de Souza Pinheiro; José Rodrigues Pinheiro Neto; Geilson Alcantara da Silva; Henrique Barros de Lima; Lorane Izabel da Silva Hage-Melim; Arlindo César Matias Pereira; José Carlos Tavares Carvalho; Sheylla Susan Moreira da Silva de Almeida

doi:10.3390/ph14090856

Pharmaceuticals (Aug 2021)

Hypoglycemic Activity of Aqueous Extract of Latex from <i>Hancornia speciosa</i> Gomes: A Study in Zebrafish and In Silico

Rosana Tomazi,
Ângela Costa Figueira,
Adriana Maciel Ferreira,
Diego Quaresma Ferreira,
Gisele Custódio de Souza,
Wandson Braamcamp de Souza Pinheiro,
José Rodrigues Pinheiro Neto,
Geilson Alcantara da Silva,
Henrique Barros de Lima,
Lorane Izabel da Silva Hage-Melim,
Arlindo César Matias Pereira,
José Carlos Tavares Carvalho,
Sheylla Susan Moreira da Silva de Almeida

Affiliations

Rosana Tomazi: Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Rede Bionorte (Ppg-Bionorte), Instituto Federal de Educação, Ciência e Tecnologia do Amapá (IFAP), Rodovia BR-210, km 03, S/n—Brasil Novo, Macapá 68909-398, AP, Brazil
Ângela Costa Figueira: Programa de Pós-Graduação em Biodiversidade e Biotecnologia da Rede Bionorte (Ppg-Bionorte), Instituto Federal de Educação, Ciência e Tecnologia do Amapá (IFAP), Rodovia BR-210, km 03, S/n—Brasil Novo, Macapá 68909-398, AP, Brazil
Adriana Maciel Ferreira: Laboratório de Pesquisa em Fármacos, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá (UNIFAP), Rod. Juscelino Kubitschek, km 02—Jardim Marco Zero, Macapá 68903-419, AP, Brazil
Diego Quaresma Ferreira: Laboratório de Pesquisa em Fármacos, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá (UNIFAP), Rod. Juscelino Kubitschek, km 02—Jardim Marco Zero, Macapá 68903-419, AP, Brazil
Gisele Custódio de Souza: Laboratório de Pesquisa em Fármacos, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá (UNIFAP), Rod. Juscelino Kubitschek, km 02—Jardim Marco Zero, Macapá 68903-419, AP, Brazil
Wandson Braamcamp de Souza Pinheiro: Laboratório de Química Industrial, Instituto de Química, Universidade Federal do Pará (UFPA), Rua. Augusto Corrêa, Guamá, 01, Belém 66075-110, AP, Brazil
José Rodrigues Pinheiro Neto: Laboratório de Química Industrial, Instituto de Química, Universidade Federal do Pará (UFPA), Rua. Augusto Corrêa, Guamá, 01, Belém 66075-110, AP, Brazil
Geilson Alcantara da Silva: Laboratório de Química Industrial, Instituto de Química, Universidade Federal do Pará (UFPA), Rua. Augusto Corrêa, Guamá, 01, Belém 66075-110, AP, Brazil
Henrique Barros de Lima: Laboratório de Química Medicinal, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá (UNIFAP), Rod. Juscelino Kubitschek, km 02—Jardim Marco Zero, Macapá 68903-419, AP, Brazil
Lorane Izabel da Silva Hage-Melim: Laboratório de Química Medicinal, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá (UNIFAP), Rod. Juscelino Kubitschek, km 02—Jardim Marco Zero, Macapá 68903-419, AP, Brazil
Arlindo César Matias Pereira: Laboratório de Pesquisa em Fármacos, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá (UNIFAP), Rod. Juscelino Kubitschek, km 02—Jardim Marco Zero, Macapá 68903-419, AP, Brazil
José Carlos Tavares Carvalho: Laboratório de Pesquisa em Fármacos, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá (UNIFAP), Rod. Juscelino Kubitschek, km 02—Jardim Marco Zero, Macapá 68903-419, AP, Brazil
Sheylla Susan Moreira da Silva de Almeida: Laboratório de Farmacognosia e Fitoquímica, Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá (UNIFAP), Rod. Juscelino Kubitschek, km 02—Jardim Marco Zero, Macapá 68903-419, AP, Brazil

DOI: https://doi.org/10.3390/ph14090856
Journal volume & issue: Vol. 14, no. 9
p. 856

Abstract

Read online

Hancornia speciosa Gomes is a tree native to Brazil and has therapeutic potential for several diseases. Ethnopharmacological surveys have reported that the plant is used as a hypoglycemic agent and to lose weight. This study aimed to evaluate the effects of the aqueous extract from H. speciosa latex (LxHs) in a zebrafish model of diabetes. The extract was evaluated through high-performance thin-layer chromatography (HTPLC), nuclear magnetic resonance (NMR), and Fourier-transform infrared spectroscopy (FT-IR). We then tested treatments with LxHs (500, 1000, and 1500 mg/kg) by assessing blood glucose levels in alloxan-induced diabetic animals, and metformin was used as a control. The toxicity was evaluated through histopathology of the pancreas and biochemical assessment of serum levels of AST, ALT, creatinine, and urea. The extract was also assessed for acute toxicity through several parameters in embryos and adult animals. Finally, we performed in silico analysis through the SEA server and docking using the software GOLD. The phytochemical study showed the compounds cornoside, dihydrocornoide, and 1-O-methyl-myoinositol (bornesitol). The treatment with all doses of LxHs significantly decreased alloxan-induced hyperglycemia without any significant histological or biochemical abnormalities. No significant frequency of teratogenesis was observed in the embryos exposed to the extract, and no significant behavioral changes or deaths were observed in adult animals. In silico, the results showed a potential interaction between inositol and enzymes involved in carbohydrates’ metabolism. Overall, the results show a hypoglycemic activity of the extract in vivo, with no apparent toxicity. The computational studies suggest this could be at least partially due to the presence of bornesitol, since inositols can interact with carbohydrates’ enzymes.

Published in Pharmaceuticals

ISSN: 1424-8247 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Pharmacy and materia medica
Website: http://www.mdpi.com/journal/pharmaceuticals/

About the journal

Abstract

Keywords