Nature Communications (Mar 2022)
First-in-human immunoPET imaging of HIV-1 infection using 89Zr-labeled VRC01 broadly neutralizing antibody
- Denis R. Beckford-Vera,
- Robert R. Flavell,
- Youngho Seo,
- Enrique Martinez-Ortiz,
- Maya Aslam,
- Cassandra Thanh,
- Emily Fehrman,
- Marion Pardons,
- Shreya Kumar,
- Amelia N. Deitchman,
- Vahid Ravanfar,
- Brailee Schulte,
- I-Wei Katherine Wu,
- Tony Pan,
- Jacqueline D. Reeves,
- Christopher C. Nixon,
- Nikita S. Iyer,
- Leonel Torres,
- Sadie E. Munter,
- Tony Hyunh,
- Christos J. Petropoulos,
- Rebecca Hoh,
- Benjamin L. Franc,
- Lucio Gama,
- Richard A. Koup,
- John R. Mascola,
- Nicolas Chomont,
- Steven G. Deeks,
- Henry F. VanBrocklin,
- Timothy J. Henrich
Affiliations
- Denis R. Beckford-Vera
- Department of Radiology and Biomedical Imaging, University of California San Francisco
- Robert R. Flavell
- Department of Radiology and Biomedical Imaging, University of California San Francisco
- Youngho Seo
- Department of Radiology and Biomedical Imaging, University of California San Francisco
- Enrique Martinez-Ortiz
- Division of HIV, Infectious Diseases and Global Medicine, University of California San Francisco
- Maya Aslam
- Department of Radiology and Biomedical Imaging, University of California San Francisco
- Cassandra Thanh
- Division of Experimental Medicine, University of California San Francisco
- Emily Fehrman
- Division of HIV, Infectious Diseases and Global Medicine, University of California San Francisco
- Marion Pardons
- Department of Microbiology, Infectiology and Immunology, Centre de Recherche du CHUM, Université de Montréal
- Shreya Kumar
- Division of Experimental Medicine, University of California San Francisco
- Amelia N. Deitchman
- Department of Clinical Pharmacy, University of California
- Vahid Ravanfar
- Department of Radiology and Biomedical Imaging, University of California San Francisco
- Brailee Schulte
- Department of Radiology and Biomedical Imaging, University of California San Francisco
- I-Wei Katherine Wu
- Department of Radiology and Biomedical Imaging, University of California San Francisco
- Tony Pan
- Division of Experimental Medicine, University of California San Francisco
- Jacqueline D. Reeves
- Division of Experimental Medicine, University of California San Francisco
- Christopher C. Nixon
- Division of Experimental Medicine, University of California San Francisco
- Nikita S. Iyer
- Division of Experimental Medicine, University of California San Francisco
- Leonel Torres
- Division of Experimental Medicine, University of California San Francisco
- Sadie E. Munter
- Division of Experimental Medicine, University of California San Francisco
- Tony Hyunh
- Department of Radiology and Biomedical Imaging, University of California San Francisco
- Christos J. Petropoulos
- Monogram Biosciences, Inc., Laboratory Corporation of America, South San Francisco
- Rebecca Hoh
- Division of HIV, Infectious Diseases and Global Medicine, University of California San Francisco
- Benjamin L. Franc
- Department of Radiology, Stanford University
- Lucio Gama
- Vaccine Research Center, National Institute for Allergy and Infectious Diseases, National Institutes of Health
- Richard A. Koup
- Vaccine Research Center, National Institute for Allergy and Infectious Diseases, National Institutes of Health
- John R. Mascola
- Vaccine Research Center, National Institute for Allergy and Infectious Diseases, National Institutes of Health
- Nicolas Chomont
- Department of Microbiology, Infectiology and Immunology, Centre de Recherche du CHUM, Université de Montréal
- Steven G. Deeks
- Division of HIV, Infectious Diseases and Global Medicine, University of California San Francisco
- Henry F. VanBrocklin
- Department of Radiology and Biomedical Imaging, University of California San Francisco
- Timothy J. Henrich
- Division of Experimental Medicine, University of California San Francisco
- DOI
- https://doi.org/10.1038/s41467-022-28727-5
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 15
Abstract
Here, the authors apply positron emission tomography (PET) imaging to visualize HIV tissue-wide burden in infected individuals using a radiolabeled broadly neutralizing antibody, 89Zr-VRC01, and show that PET tracer lymph node uptake positively correlates with HIV protein levels measured directly from cells obtained from these tissues. This strategy may allow non-invasive characterization of residual HIV infection in the setting of therapeutic interventions.
