Assessment of Sprague Dawley rats as biomodel in two antigenotoxicity assays

Abstract

The discovery of new drugs with antigenotoxic effect constitutes in our days a prioritized line of research. In this work was evaluated the response of Sprague Dawley rats (both sexes) against cyclophosphamide and bleomycin by means of alkaline comet assay of peripheral blood leukocytes and micronuclei in bone marrow cells assay as antigenotoxicy biomodel. Higher induction of the strand breaks (SB) and alkali-labile sites formation on DNA damage were obtained with the use of the cyclophosphamide and bleomycin, both in the design of 48 and 24 hr administration before the euthanasia. In the mironucleis assay was obtained high results of induction the micronucleus formation in bone marrow cells with cyclophosphamide was administered 48 and 24 hr before euthanasia in both sexes. These results are useful in studies of drugs evaluation that they have not been explored in to the in vivo antigenotoxicity environment. [Vet. World 2011; 4(10.000): 455-460]

Keywords

• Sprague Dawley
• rats
• biomodel
• antigenotoxicity assays
• micronucleus assay
• comet assay