Nature Communications (Jun 2022)
Wiskott-Aldrich syndrome protein forms nuclear condensates and regulates alternative splicing
- Baolei Yuan,
- Xuan Zhou,
- Keiichiro Suzuki,
- Gerardo Ramos-Mandujano,
- Mengge Wang,
- Muhammad Tehseen,
- Lorena V. Cortés-Medina,
- James J. Moresco,
- Sarah Dunn,
- Reyna Hernandez-Benitez,
- Tomoaki Hishida,
- Na Young Kim,
- Manal M. Andijani,
- Chongwei Bi,
- Manching Ku,
- Yuta Takahashi,
- Jinna Xu,
- Jinsong Qiu,
- Ling Huang,
- Christopher Benner,
- Emi Aizawa,
- Jing Qu,
- Guang-Hui Liu,
- Zhongwei Li,
- Fei Yi,
- Yanal Ghosheh,
- Changwei Shao,
- Maxim Shokhirev,
- Patrizia Comoli,
- Francesco Frassoni,
- John R. Yates,
- Xiang-Dong Fu,
- Concepcion Rodriguez Esteban,
- Samir Hamdan,
- Mo Li,
- Juan Carlos Izpisua Belmonte
Affiliations
- Baolei Yuan
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- Xuan Zhou
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- Keiichiro Suzuki
- Gene Expression Laboratory, Salk Institute for Biological Studies
- Gerardo Ramos-Mandujano
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- Mengge Wang
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- Muhammad Tehseen
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- Lorena V. Cortés-Medina
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- James J. Moresco
- Department of Cell Biology, Scripps Research Institute
- Sarah Dunn
- The Waitt Advanced Biophotonics Core Facility, Salk Institute for Biological Studies
- Reyna Hernandez-Benitez
- Gene Expression Laboratory, Salk Institute for Biological Studies
- Tomoaki Hishida
- Gene Expression Laboratory, Salk Institute for Biological Studies
- Na Young Kim
- Gene Expression Laboratory, Salk Institute for Biological Studies
- Manal M. Andijani
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- Chongwei Bi
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- Manching Ku
- Next-generation sequencing core, Salk Institute for Biological Studies
- Yuta Takahashi
- Gene Expression Laboratory, Salk Institute for Biological Studies
- Jinna Xu
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- Jinsong Qiu
- Department of Cellular & Molecular Medicine, University of California at San Diego
- Ling Huang
- Integrative Genomics and Bioinformatics Core, Salk Institute for Biological Studies
- Christopher Benner
- Integrative Genomics and Bioinformatics Core, Salk Institute for Biological Studies
- Emi Aizawa
- Gene Expression Laboratory, Salk Institute for Biological Studies
- Jing Qu
- State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences
- Guang-Hui Liu
- State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences
- Zhongwei Li
- Gene Expression Laboratory, Salk Institute for Biological Studies
- Fei Yi
- Gene Expression Laboratory, Salk Institute for Biological Studies
- Yanal Ghosheh
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- Changwei Shao
- Department of Cellular & Molecular Medicine, University of California at San Diego
- Maxim Shokhirev
- Integrative Genomics and Bioinformatics Core, Salk Institute for Biological Studies
- Patrizia Comoli
- Pediatric Hematology/Oncology and Cell Factory, Fondazione IRCCS Policlinico San Matteo
- Francesco Frassoni
- Department of Research Laboratories and Director of Center for Stem Cell and Cell Therapy, Instituto G. Gaslini Children Hospital Scientific Institute
- John R. Yates
- Department of Cell Biology, Scripps Research Institute
- Xiang-Dong Fu
- Department of Cellular & Molecular Medicine, University of California at San Diego
- Concepcion Rodriguez Esteban
- Gene Expression Laboratory, Salk Institute for Biological Studies
- Samir Hamdan
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- Mo Li
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- Juan Carlos Izpisua Belmonte
- Bioscience Program, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology (KAUST)
- DOI
- https://doi.org/10.1038/s41467-022-31220-8
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 20
Abstract
Wiskott-Aldrich syndrome is caused by mutations in WASP, but the underlying mechanisms remain to be explored. Here the authors reveal that WASP deficiency results in aberrant RNA splicing, and that WASP regulates the transcription of splicing factor genes and co-transcriptional RNA splicing via a phase-separation process that involves splicing factors and nascent RNA.
