Molecular strategies for the differentiation and identification of local E. coli isolated from chicken: I. Characterization of protein profile.

Abstract

In this study five serotypes of E. coli were isolated from chickens and identified as O1, O2, O6, O78 and O126 out of 33 isolates derived from a total of 60 samples. SDS-PAGE revealed that four proteins were characteristic and shared in all these serotypes at the molecular weight of 21, 30, 55 and 74 kDa of which 55 and 74 kDa proteins were fully reacted with the antisera against E. coli in the western blot. Other proteins are present but varied from one serotype to another.

Keywords

• molecular; strategies; differentiation; identification; local; e. coli; isolated; chicken; characterization; protein; profile