Applied Microscopy (Jul 2021)

Multispectral intravital microscopy for simultaneous bright-field and fluorescence imaging of the microvasculature

  • Barry G. H. Janssen,
  • Mohamadreza Najiminaini,
  • Yan Min Zhang,
  • Parsa Omidi,
  • Jeffrey J. L. Carson

DOI
https://doi.org/10.1186/s42649-021-00059-6
Journal volume & issue
Vol. 51, no. 1
pp. 1 – 12

Abstract

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Abstract Intravital video microscopy permits the observation of microcirculatory blood flow. This often requires fluorescent probes to visualize structures and dynamic processes that cannot be observed with conventional bright-field microscopy. Conventional light microscopes do not allow for simultaneous bright-field and fluorescent imaging. Moreover, in conventional microscopes, only one type of fluorescent label can be observed. This study introduces multispectral intravital video microscopy, which combines bright-field and fluorescence microscopy in a standard light microscope. The technique enables simultaneous real-time observation of fluorescently-labeled structures in relation to their direct physical surroundings. The advancement provides context for the orientation, movement, and function of labeled structures in the microcirculation.

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