Gene activation in <i>Caenorhabditis elegans</i> using the <i>Campylobacter jejuni</i> CRISPR-Cas9 feeding system

Abstract

AbstractClustered regularly interspaced palindromic repeats-based activation system, a powerful genetic manipulation technology, can modulate endogenous gene transcription in various organisms through fusing nuclease-deficient Cas9 to transcriptional regulatory domains. At present, this clustered regularly interspaced palindromic repeats-based activation system has been applied to activate gene expression by microinjection manner in CaenorhabditiselegansCampylobacter jejunC. elegansCjC. elegans