Assessing the Efficacy of Dextran and Dextransucrase in Modulating MCF-7 Breast Cancer Cell Activity

Abstract

Abstract Tumor development is a complex process involving abnormal cell proliferation within an organism. This study aims to understand tumors, their categorization, formation methods, and effects on human health. Tumor growth is influenced by genetic, environmental, and lifestyle factors. Dextran, a glucose-based polysaccharide, has been used in medical applications for blood plasma substitutes and antithrombotic agents. Researchers are interested in its potential therapeutic applications due to its biocompatibility and unique properties. Dextransucrase, a glucosyltransferase, plays a vital role in synthesizing glucan polymers. Advancements in cancer research reveal the nuanced roles of biopolymers like dextran in tumor biology, drug delivery, and cancer therapy. In this study MCF-7 cell line maintained in MEM, reseeded twice a week, incubated at 37°C. The MTT cell viability assay was conducted on 96-well plates to assess cytotoxic effects. Cell lines were seeded, treated with the tested compound, and measured after 72 hours. After solubilization, absorbency was determined using a microplate reader at 492 nm. The results showed A decrease in cell viability was observed with decreasing Dextran concentration, with a negative correlation between concentration and cell viability. A P-value of 0.001 indicates statistically significant effects on MCF-7 cell cytotoxicity, rejecting the null hypothesis that Dextran has no effect. Dextransucrase exhibited a dose-dependent cytotoxicity effect on MCF-7 cells, with a significant effect at the highest concentration (1000) and a negative correlation at the lowest concentration (31.25). The P-value of 0.001 was below the 0.05 threshold, rejecting the null hypothesis. According to the results of cell viability, the highest concentration (1000) had the lowest viability, while the lowest concentration (31.25) had the highest. The data supports the cytotoxic potential of Dextransucrase in a dose-dependent manner.

Keywords

• angiogenesis, cytotoxic, IC50, Neoplasms and viability