Evaluation of Genetic Damage and Antigenotoxic Effect of Ascorbic Acid in Erythrocytes of <i>Orochromis niloticus</i> and <i>Ambystoma mexicanum</i> Using Migration Groups as a Parameter

Abstract

The comet assay system is an efficient method used to assess DNA damage and repair; however, it currently provides the average result and, unfortunately, the heterogeneity of DNA damage loses relevance. To take advantage of this heterogeneity, migration groups (MGs) of cell comets can be formed. In this study, genetic damage was quantified in erythrocytes of Oreochromis niloticus and Ambystoma mexicanum exposed to ethyl methanesulfonate (ethyl methanesulfonate (EMS) 2.5, 5, and 10 mM over two hours) and ultraviolet C radiation (UV-C) for 5, 10, and 15 min using the tail length, tail moment, and migration group parameters. Additionally, blood cells were exposed to UV-C radiation for 5 min and treated post-treatment at 5, 10, and 15 mM ascorbic acid (AA) for two hours. With the MG parameter, it was possible to observe variations in the magnitude of genetic damage. Our data indicate that MGs help to detect basal and induced genetic damage or damage reduction with approximately the same efficiency of the tail length and tail moment parameters. MGs can be a complementary parameter used to assess DNA integrity in species exposed to mutagens.

Keywords

• comet assay
• migration groups
• bioassays
• genetic damage
• ascorbic acid