Data on biochemical fluxes generated from biofabricated enzyme complexes assembled through engineered tags and microbial transglutaminase

Narendranath Bhokisham; Haig Pakhchanian; David Quan; Tanya Tschirhart; Chen-Yu Tsao; Gregory F. Payne; William E. Bentley

Data in Brief (Sep 2016)

Data on biochemical fluxes generated from biofabricated enzyme complexes assembled through engineered tags and microbial transglutaminase

Narendranath Bhokisham,
Haig Pakhchanian,
David Quan,
Tanya Tschirhart,
Chen-Yu Tsao,
Gregory F. Payne,
William E. Bentley

Affiliations

Narendranath Bhokisham: Biological Sciences Graduate Program - College of Computer, Mathematical and Natural Sciences, University of Maryland, 4066 Campus Drive, College Park, MD 20742, United States; Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States
Haig Pakhchanian: Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States
David Quan: Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States; Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States
Tanya Tschirhart: Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States; Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States
Chen-Yu Tsao: Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States; Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States
Gregory F. Payne: Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States; Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States
William E. Bentley: Biological Sciences Graduate Program - College of Computer, Mathematical and Natural Sciences, University of Maryland, 4066 Campus Drive, College Park, MD 20742, United States; Institute of Bioscience and Biotechnology Research, University of Maryland, College Park, 5115 Plant Science and Landscape Architecture Building, College Park, MD 20742, United States; Fischell Department of Bioengineering, University of Maryland, Room 3122, Jeong H. Kim Engineering Building (Bldg. #225), College Park, MD 20742, United States; Corresponding author at: Fischell Department of Bioengineering, Room 3122, Jeong H.Kim Engineering Building (Bldg.#225), University of Maryland, College Park, MD 20742, United States.

Journal volume & issue: Vol. 8
pp. 1031 – 1035

Abstract

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Data presented is related to an article titled “Modular construction of multi-subunit protein complexes using engineered tags and microbial transglutaminase” (Bhokisham et al., 2016) [1]. In this article, we have presented western blot and flux data associated with assembly of Pfs–LuxS enzyme complexes on beads using uni-tagged and bi-tagged LuxS enzymes. We have also presented biochemical flux following changes in enzyme stoichiometries. We covalently coupled a Pfs-LuxS complex with Protein G, an antibody binding non-enzyme component and directed these complexes to the surfaces of bacterial cells via anti-Escherichia coli antibodies. Fluorescence microscopy images represented the altered behavior of bacterial cells in response to the autoinducer-2 that is synthesized by the Protein G-enzyme complexes. Keywords: Biofabrication, Metabolic flux, Engineered tags, Transglutaminase, Quorum sensing

Published in Data in Brief

ISSN: 2352-3409 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Medicine (General): Computer applications to medicine. Medical informatics; Science: Science (General)
Website: http://www.journals.elsevier.com/data-in-brief/

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