Improvement of methods for diagnosing reproductive pathologies in cattle

Abstract

The object of the study was Holsteinized cows with a productivity of 13300 kg. The subject of the study was the blood and urine of dry cows in which puerperal paresis and afterbirth retention were recorded during childbirth. A Mindrey BC-2800 Vet. hematology analyzer, Germany, a Chem Well combi Models 2902 and 2910 (USA) analyzer was used for blood biochemistry, and a LabUReader Plus 2 analyzer (Hungary) was used for urine analysis. Placenta samples were obtained at birth and subjected to standard histological procedures using immunohistochemical and immunofluorographic-intensive methods. The study provides the first evidence that cows in the last stages of gestation and during the period of weaning of the newborn from the birth canal showed a decrease in IRI, an increase in ALP and ceruloplasmin, which indicates the association of these indicators with reproductive pathology. The increased content of progesterone and cortisol in the blood of animals is associated with the loss of the ability of endometrial target cells to receive sex hormones during childbirth, and their readiness to manifest further reproductive function. In the tissues of the uterine part of the placenta of a cow during pathological childbirth, degradation of the connective tissue matrix of the crypts of the caruncles was established, which indicates the structurelessness of the interstitial substance of the uterine crypts of the caruncles. Translocation of MMP-1 and MMP-2 was observed in the space between the villi of the allantochorion cotyledon and the epithelium of the crypts of caruncles, as well as in the cytoplasm of syncytiotrophoblasts and trophoblasts.

Keywords

• pregnancy
• delivery of cows
• retention of the afterbirth
• puerperal paresis
• blood