Nature Communications (Jul 2017)

Generation and comparison of CRISPR-Cas9 and Cre-mediated genetically engineered mouse models of sarcoma

  • Jianguo Huang,
  • Mark Chen,
  • Melodi Javid Whitley,
  • Hsuan-Cheng Kuo,
  • Eric S. Xu,
  • Andrea Walens,
  • Yvonne M. Mowery,
  • David Van Mater,
  • William C. Eward,
  • Diana M. Cardona,
  • Lixia Luo,
  • Yan Ma,
  • Omar M. Lopez,
  • Christopher E. Nelson,
  • Jacqueline N. Robinson-Hamm,
  • Anupama Reddy,
  • Sandeep S. Dave,
  • Charles A. Gersbach,
  • Rebecca D. Dodd,
  • David G. Kirsch

DOI
https://doi.org/10.1038/ncomms15999
Journal volume & issue
Vol. 8, no. 1
pp. 1 – 11

Abstract

Read online

Site-specific recombination and CRISPR-Cas9 have been used to generate genetically engineered mouse models of cancer. Here the authors compare sarcomas generated using both systems and see similar genetic and cellular phenotypes, suggesting CRISPR-Cas9 can be used to rapidly generate sarcoma models.