Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors

Abstract

Alkanethiol self-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposed as a novel probe design in electrochemical biosensors. This paper investigates the optimization of PNA probe by using two different concentrations of SAMs, immobilization temperature, and two-stage immobilization process. Alkanethiol self-assembled monolayers with PNA probe immobilization and cDNA (complementary DNA) hybridization were measured via AC voltammetry method. The small negative shift in formal potential (-17 mV) was occurred after cDNA hybridization at high ionic strength concentration.

Keywords

• alkanethiol self-assembled monolayers
• pna
• electrochemical biosensors
• ac voltammetry
• alkanetiol kendiliğinden oluşan tek katmanlar
• pna
• elektrokimyasal biyosensörler
• ac voltametre