Various methods for isolating DNA from fruiting bodies on the example of Pleurotus pulmonarius (higher basidiomycetes) of the Novosibirsk Region for barcoding of edible and medicinal mushrooms

Abstract

We conducted experiments to increase the concentration of extracted DNA from the fruiting bodies of this species using various lysis buffers and extraction time. In general, DNA isolation according to the protocol using SDS made it possible to obtain higher concentrations thereof, as in the case of increasing the extraction time from 1 to 24 hours and using additional purification with chloroform.