Frontiers in Cell and Developmental Biology (Sep 2021)
A Three-Dimensional Imaging Method for the Quantification and Localization of Dynamic Cell Tracking Posttransplantation
- Fengfeng Lu,
- Fengfeng Lu,
- Fengfeng Lu,
- Xin Pan,
- Xin Pan,
- Xin Pan,
- Wencheng Zhang,
- Wencheng Zhang,
- Wencheng Zhang,
- Xin Su,
- Xin Su,
- Xin Su,
- Yuying Gu,
- Hua Qiu,
- Hua Qiu,
- Shengwei Shen,
- Shengwei Shen,
- Changcheng Liu,
- Changcheng Liu,
- Changcheng Liu,
- Wei Liu,
- Wei Liu,
- Wei Liu,
- Xicheng Wang,
- Xicheng Wang,
- Xicheng Wang,
- Zhenzhen Zhan,
- Zhongmin Liu,
- Zhongmin Liu,
- Zhongmin Liu,
- Zhongmin Liu,
- Zhiying He,
- Zhiying He,
- Zhiying He
Affiliations
- Fengfeng Lu
- Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
- Fengfeng Lu
- Shanghai Engineering Research Center of Stem Cells Translational Medicine, Shanghai, China
- Fengfeng Lu
- Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai, China
- Xin Pan
- Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
- Xin Pan
- Shanghai Engineering Research Center of Stem Cells Translational Medicine, Shanghai, China
- Xin Pan
- Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai, China
- Wencheng Zhang
- Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
- Wencheng Zhang
- Shanghai Engineering Research Center of Stem Cells Translational Medicine, Shanghai, China
- Wencheng Zhang
- Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai, China
- Xin Su
- Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
- Xin Su
- Shanghai Engineering Research Center of Stem Cells Translational Medicine, Shanghai, China
- Xin Su
- Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai, China
- Yuying Gu
- Department of Cardiology, Shanghai East Hospital, Tongji University, Shanghai, China
- Hua Qiu
- Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
- Hua Qiu
- The First Affiliated Hospital of Nanchang University, Nanchang, China
- Shengwei Shen
- Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
- Shengwei Shen
- Department of Hepatobiliary and Pancreatic Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, China
- Changcheng Liu
- Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
- Changcheng Liu
- Shanghai Engineering Research Center of Stem Cells Translational Medicine, Shanghai, China
- Changcheng Liu
- Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai, China
- Wei Liu
- Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
- Wei Liu
- Shanghai Engineering Research Center of Stem Cells Translational Medicine, Shanghai, China
- Wei Liu
- Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai, China
- Xicheng Wang
- Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
- Xicheng Wang
- Shanghai Engineering Research Center of Stem Cells Translational Medicine, Shanghai, China
- Xicheng Wang
- Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai, China
- Zhenzhen Zhan
- Institute of Heart Failure, Shanghai East Hospital, Tongji University, Shanghai, China
- Zhongmin Liu
- Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
- Zhongmin Liu
- Shanghai Engineering Research Center of Stem Cells Translational Medicine, Shanghai, China
- Zhongmin Liu
- Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai, China
- Zhongmin Liu
- Institute of Heart Failure, Shanghai East Hospital, Tongji University, Shanghai, China
- Zhiying He
- Institute for Regenerative Medicine, Shanghai East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai, China
- Zhiying He
- Shanghai Engineering Research Center of Stem Cells Translational Medicine, Shanghai, China
- Zhiying He
- Shanghai Institute of Stem Cell Research and Clinical Translation, Shanghai, China
- DOI
- https://doi.org/10.3389/fcell.2021.698795
- Journal volume & issue
-
Vol. 9
Abstract
Cell transplantation has been proposed as a promising therapeutic strategy for curing the diseases requiring tissue repairing and functional restoration. A preclinical method to systematically evaluate the fates of donor cells in recipients, spatially and temporally, is demanded for judging therapeutic potentials for the particularly designed cell transplantation. Yet, the dynamic cell tracking methodology for tracing transplanted cells in vivo is still at its early phase. Here, we created a practical protocol for dynamically tracking cell via a three-dimensional (3D) technique which enabled us to localize, quantify, and overall evaluate the transplanted hepatocytes within a liver failure mouse model. First, the capacity of 3D bioluminescence imaging for quantifying transplanted hepatocytes was defined. Images obtained from the 3D bioluminescence imaging module were then combined with the CT scanner to reconstruct structure images of host mice. With those reconstructed images, precise locations of transplanted hepatocytes in the liver of the recipient were dynamically monitored. Immunohistochemistry staining of transplanted cells, and the serology assay of liver panel of the host mice were applied to verify the successful engraftment of donor cells in the host livers. Our protocol was practical for evaluating the engraftment efficiency of donor cells at their preclinical phases, which is also applicable as a referable standard for studying the fates of other transplanted cells, such as stem cell-derived cell types, during preclinical studies with cell transplantation therapy.
Keywords
- cell transplantation
- in vivo tracking
- bioluminescence imaging (BLI)
- computer tomography (CT)
- cell distribution
