Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions

Pawel Nowialis; Katarina Lopusna; Jana Opavska; Staci L. Haney; Ajay Abraham; Peike Sheng; Alberto Riva; Amarnath Natarajan; Olga Guryanova; Melanie Simpson; Ryan Hlady; Mingyi Xie; Rene Opavsky

doi:10.1038/s41467-019-12355-7

Nature Communications (Sep 2019)

Catalytically inactive Dnmt3b rescues mouse embryonic development by accessory and repressive functions

Pawel Nowialis,
Katarina Lopusna,
Jana Opavska,
Staci L. Haney,
Ajay Abraham,
Peike Sheng,
Alberto Riva,
Amarnath Natarajan,
Olga Guryanova,
Melanie Simpson,
Ryan Hlady,
Mingyi Xie,
Rene Opavsky

Affiliations

Pawel Nowialis: Department of Anatomy and Cell Biology, University of Florida College of Medicine
Katarina Lopusna: Department of Anatomy and Cell Biology, University of Florida College of Medicine
Jana Opavska: Department of Anatomy and Cell Biology, University of Florida College of Medicine
Staci L. Haney: Department of Internal Medicine, University of Nebraska Medical Center
Ajay Abraham: Department of Anatomy and Cell Biology, University of Florida College of Medicine
Peike Sheng: Department of Biochemistry and Molecular Biology, University of Florida College of Medicine
Alberto Riva: ICBR Bioinformatics, Cancer and Genetics Research Complex, University of Florida
Amarnath Natarajan: University of Nebraska Medical Center, The Eppley Institute for Research in Cancer and Allied Diseases, Fred & Pamela Buffett Cancer Center, 986805 Nebraska Medical Center
Olga Guryanova: Department of Pharmacology and Therapeutics, University of Florida College of Medicine
Melanie Simpson: Molecular and Structural Biochemistry, NC State University
Ryan Hlady: Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic
Mingyi Xie: UF Health Cancer Center, University of Florida
Rene Opavsky: Department of Anatomy and Cell Biology, University of Florida College of Medicine

DOI: https://doi.org/10.1038/s41467-019-12355-7
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 16

Abstract

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The family of DNA methyltransferases (Dnmts) consists of four catalytically active enzymes that catalyze DNA methylation and also play a role as transcriptional regulators. Here the authors show that catalytically inactive Dnmt3b rescues a majority of methylation and expression changes in the absence of Dnmt3b during mouse embryonic development.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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