Nature Communications (Aug 2018)

Revealing circadian mechanisms of integration and resilience by visualizing clock proteins working in real time

  • Tetsuya Mori,
  • Shogo Sugiyama,
  • Mark Byrne,
  • Carl Hirschie Johnson,
  • Takayuki Uchihashi,
  • Toshio Ando

DOI
https://doi.org/10.1038/s41467-018-05438-4
Journal volume & issue
Vol. 9, no. 1
pp. 1 – 13

Abstract

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The circadian clock proteins KaiA, KaiB, and KaiC reconstitute a circa-24 h oscillation of KaiC phosphorylation in vitro. Here the authors use high-speed atomic force microscopy to visualize in real time and quantify the dynamic interactions of KaiA with KaiC on the sub-second timescale to discover mechanisms of oscillatory resilience.