Strain specificity of lactobacilli with promoted colonization by galactooligosaccharides administration in protecting intestinal barriers during Salmonella infection

Yujun Wu; Xiangyu Zhang; Xiaoyi Liu; Yi Li; Dandan Han; Yu Pi; Melanie A. Whitmore; Xingmiao Lu; Guolong Zhang; Jinkai Zheng; Junjun Wang

Journal of Advanced Research (Feb 2024)

Strain specificity of lactobacilli with promoted colonization by galactooligosaccharides administration in protecting intestinal barriers during Salmonella infection

Yujun Wu,
Xiangyu Zhang,
Xiaoyi Liu,
Yi Li,
Dandan Han,
Yu Pi,
Melanie A. Whitmore,
Xingmiao Lu,
Guolong Zhang,
Jinkai Zheng,
Junjun Wang

Affiliations

Yujun Wu: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Xiangyu Zhang: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Xiaoyi Liu: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Yi Li: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Dandan Han: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Yu Pi: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
Melanie A. Whitmore: Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Xingmiao Lu: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Guolong Zhang: Department of Animal and Food Sciences, Oklahoma State University, Stillwater, OK 74078, USA
Jinkai Zheng: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Junjun Wang: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; Corresponding author.

Journal volume & issue: Vol. 56
pp. 1 – 14

Abstract

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Introduction: Galactooligosaccharides (GOS) are lactogenic prebiotics that exert health benefits by stimulating the growth of different Lactobacillus strains in the gastrointestinal (GI) tract. Objectives: This study aimed to investigate the mechanism of action of different GOS-enriched lactobacilli in intestinal health. Methods: Piglets and mice were supplemented with GOS to identify specific enrichment of Lactobacillus. The protective effects of individual GOS-enriched lactobacilli were investigated in Salmonella-infected mice. Macrophage depletion and transcriptome analysis were further performed to assess the involvement of macrophages and the underlying mechanisms of individual lactobacilli. An in vitro cell co-culture system was also used to evaluate the anti-adhesive and anti-invasive activities of lactobacilli against Salmonella in epithelial cells. Results: GOS markedly increased the relative abundance of three lactobacilli including L. delbrueckii, L. johnsonii, and L. reuteri in both piglets and mice. Supplementation with GOS further alleviated Salmonella infection in mice. L. delbrueckii (ATCC®BAA 365™), but not L. johnsonii or L. reuteri, enhanced propionate production in the intestinal tract and ameliorated Salmonella-induced intestinal inflammation and barrier dysfunction by suppressing the JAK2-STAT3 signaling and M1 macrophage polarization. L. johnsonii (BNCC 186110), on the other hand, inhibited Salmonella adhesion and invasion of epithelial cells through competitive exclusion. However, L. reuteri (BNCC 186135) failed to protect mice against Salmonella infection. Conclusion: GOS-enriched lactobacilli show a differential role in protecting against Salmonella-induced intestinal barrier dysfunction and inflammation. Our results provide novel insights into the mechanism of action of GOS and individual Lactobacillus strains in the control and prevention of intestinal inflammatory disorders.

Published in Journal of Advanced Research

ISSN: 2090-1232 (Print); 2090-1224 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Science (General)
Website: http://www.journals.elsevier.com/journal-of-advanced-research/

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