Revista Brasileira de Zootecnia (Dec 2011)

Correlation between sperm characteristics and testosterone in bovine seminal plasma by direct radioimmunoassay

  • Luiz Waldemar de Oliveira Souza,
  • André Furugen Cesar Andrade,
  • Eneiva Carla Carvalho Celeghini,
  • João Alberto Negrão,
  • Rubens Paes de Arruda

DOI
https://doi.org/10.1590/S1516-35982011001200015
Journal volume & issue
Vol. 40, no. 12
pp. 2721 – 2724

Abstract

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The objectives of this study were to validate a non-extractive RIA for seminal testosterone and quantify the hormone using a solid-phase commercial kit, and study the correlation between testosterone in seminal plasma and sperm characteristics. Parallelism showed a correlation index r = 0.992 (Y = -5.47 + 1.073X; R² = 0.985), indicating that the non-extractive method presented is indicated particularly for assessment of testosterone when establishing comparisons between samples. Overall mean (±SD) of testosterone level was 0.60±0.65 ng/mL. Correlation was only found between the seminal concentrations of testosterone and pH of the semen.

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