Prolidase Activity and Oxidative Stress in Patients with Glioma

Abstract

Introduction: Glioma is a glial cell tumour. Altered inflammatory responses and oxidative stress can be associated with induction of angiogenesis and uncontrolled growth of cells. Prolidase is a metalloenzyme also associated with angiogenesis and cancer. However, studies on prolidase and oxidative stress in glioma patients are limited. Aim: To observe the prolidase activity and oxidative stress markers, such as Total Oxidant Status (TOS), Total Antioxidant Status (TAS) And Oxidative Stress Index (OSI), in patients with glioma. Materials and Methods: Total 46 patients with glioma, 20 cadaver and 46 healthy volunteers of age and gender matched were selected as Cases, Control-1 and Control-2, respectively. Blood and brain tissues of respective area of cases and Control-1 were collected, while only blood was collected from Control-2. After brain tissue homogenisation and sera separation, prolidase activity and oxidative stress parameters were assessed spectrophotometrically. Results: Prolidase activity and oxidative stress was found to be significantly elevated in the glioma tissues as well as sera of the patients with glioma as compared to healthy and cadaver controls (all p<0.01). Total oxidant status and oxidative stress index were also significantly elevated in glioma tissues and sera of patients with glioma, while total antioxidant status was significantly decreased (all p<0.01). Conclusion: The present study showed that prolidase activity and oxidative stress markers such as TOS and OSI were significantly elevated in the patients with glioma, while level of TAS was decreased. Thus, it seems that elevated prolidase activity and oxidative stress markers might be associated with the pathogenesis of glioma.

Keywords

• glial cell tumour
• total antioxidant status
• total oxidant status