CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing

Abstract

Kelil, Gallo et al. develop an antibody discovery strategy, termed CellectSeq, for targeting integral membrane proteins on native cells. This strategy uses motif-based scoring and sequencing error-filtering algorithms to identify antibodies with high diversity and specificity even at extremely low abundances in NGS pools.