Frontiers in Physiology (Feb 2018)
Ginsenoside Rg1 Ameliorates Rat Myocardial Ischemia-Reperfusion Injury by Modulating Energy Metabolism Pathways
- Lin Li,
- Lin Li,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Chun-Shui Pan,
- Li Yan,
- Li Yan,
- Li Yan,
- Li Yan,
- Li Yan,
- Yuan-Chen Cui,
- Yuan-Chen Cui,
- Yuan-Chen Cui,
- Yuan-Chen Cui,
- Yuan-Chen Cui,
- Yu-Ying Liu,
- Yu-Ying Liu,
- Yu-Ying Liu,
- Yu-Ying Liu,
- Yu-Ying Liu,
- Hong-Na Mu,
- Hong-Na Mu,
- Hong-Na Mu,
- Hong-Na Mu,
- Hong-Na Mu,
- Ke He,
- Ke He,
- Ke He,
- Ke He,
- Ke He,
- Bai-He Hu,
- Bai-He Hu,
- Bai-He Hu,
- Bai-He Hu,
- Bai-He Hu,
- Xin Chang,
- Xin Chang,
- Xin Chang,
- Xin Chang,
- Xin Chang,
- Kai Sun,
- Kai Sun,
- Kai Sun,
- Kai Sun,
- Kai Sun,
- Jing-Yu Fan,
- Jing-Yu Fan,
- Jing-Yu Fan,
- Jing-Yu Fan,
- Jing-Yu Fan,
- Li Huang,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han,
- Jing-Yan Han
Affiliations
- Lin Li
- Department of Integrative Cardiology, Beijing China-Japan Friendship Hospital, Beijing, China
- Lin Li
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Chun-Shui Pan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Chun-Shui Pan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Chun-Shui Pan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Chun-Shui Pan
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Chun-Shui Pan
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Li Yan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Li Yan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Li Yan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Li Yan
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Li Yan
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Yuan-Chen Cui
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Yuan-Chen Cui
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Yuan-Chen Cui
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Yuan-Chen Cui
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Yuan-Chen Cui
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Yu-Ying Liu
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Yu-Ying Liu
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Yu-Ying Liu
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Yu-Ying Liu
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Yu-Ying Liu
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Hong-Na Mu
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Hong-Na Mu
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Hong-Na Mu
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Hong-Na Mu
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Hong-Na Mu
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Ke He
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Ke He
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Ke He
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Ke He
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Ke He
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Bai-He Hu
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Bai-He Hu
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Bai-He Hu
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Bai-He Hu
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Bai-He Hu
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Xin Chang
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Xin Chang
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Xin Chang
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Xin Chang
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Xin Chang
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Kai Sun
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Kai Sun
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Kai Sun
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Kai Sun
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Kai Sun
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Jing-Yu Fan
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Jing-Yu Fan
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Jing-Yu Fan
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Jing-Yu Fan
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Jing-Yu Fan
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Li Huang
- Department of Integrative Cardiology, Beijing China-Japan Friendship Hospital, Beijing, China
- Jing-Yan Han
- Tasly Microcirculation Research Center, Peking University Health Science Center, Beijing, China
- Jing-Yan Han
- Key Laboratory of Microcirculation, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Jing-Yan Han
- Key Laboratory of Stasis and Phlegm, State Administration of Traditional Chinese Medicine of the People's Republic of China, Beijing, China
- Jing-Yan Han
- Beijing Microvascular Institute of Integration of Chinese and Western Medicine, Beijing, China
- Jing-Yan Han
- State Key Laboratory of Core Technology in Innovative Chinese Medicine, Beijing, China
- Jing-Yan Han
- Department of Integration of Chinese and Western Medicine, School of Basic Medical Sciences, Peking University, Beijing, China
- DOI
- https://doi.org/10.3389/fphys.2018.00078
- Journal volume & issue
-
Vol. 9
Abstract
As a major ingredient of Radix ginseng, ginsenoside Rg1 (Rg1) has been increasingly recognized to benefit the heart condition, however, the rationale behind the role is not fully understood. In vitro study in H9c2 cardiomyocytes has shown the potential of Rg1 to increase ATP content in the cells. We thus speculated that the protective effect of Rg1 on heart ischemia and reperfusion (I/R) injury implicates energy metabolism regulation. The present study was designed to verify this speculation. Male Sprague-Dawley rats were subjected to 30 min of occlusion of left coronary anterior descending artery followed by reperfusion for 90 min. Rg1 (5 mg/kg/h) was continuously administrated intravenously 30 min before occlusion until the end of reperfusion. Myocradial blood flow and heart function were monitored over the period of I/R. Myocardial infarct size, structure and apoptosis, energy metabolism, and change in RhoA signaling pathway were evaluated 90 min after reperfusion. Binding of Rg1 to RhoA was assessed using Surface Plasmon Resonance (SPR). Rg1 prevented I/R-elicited insults in myocardium, including myocardial infarction and apoptosis, decreased myocardial blood flow (MBF) and heart function, and alteration in myocardium structure. Rg1 restored the production of ATP in myocardium after I/R. Rg1 was able to bind to RhoA and down-regulate the activity of RhoA signaling pathway. These results indicated that Rg1 had protective potential against I/R-induced myocardial injury, which may be related to inhibiting myocardial apoptosis and modulating energy metabolism through binding to RhoA.
Keywords