Analysis of magnetic resonance contrast agent entrapment following reversible electroporation in vitro

Abstract

Administering gadolinium-based contrast agent before electroporation allows the contrast agent to enter the cells and enables MRI assessment of reversibly electroporated regions. The aim of this study was evaluation of contrast agent entrapment in Chinese hamster ovary (CHO) cells and comparison of these results with those determined by standard in vitro methods for assessing cell membrane permeability, cell membrane integrity and cell survival following electroporation.

Keywords

• electroporation
• membrane permeabilization
• magnetic resonance contrast agent
• t1 relaxometry