Architecture of a SARS-CoV-2 mini replication and transcription complex

Liming Yan; Ying Zhang; Ji Ge; Litao Zheng; Yan Gao; Tao Wang; Zhihui Jia; Haofeng Wang; Yucen Huang; Mingyu Li; Quan Wang; Zihe Rao; Zhiyong Lou

doi:10.1038/s41467-020-19770-1

Nature Communications (Nov 2020)

Architecture of a SARS-CoV-2 mini replication and transcription complex

Liming Yan,
Ying Zhang,
Ji Ge,
Litao Zheng,
Yan Gao,
Tao Wang,
Zhihui Jia,
Haofeng Wang,
Yucen Huang,
Mingyu Li,
Quan Wang,
Zihe Rao,
Zhiyong Lou

Affiliations

Liming Yan: MOE Key Laboratory of Protein Science, School of Medicine, Tsinghua University
Ying Zhang: School of Life Sciences, Tsinghua University
Ji Ge: School of Life Sciences, Tsinghua University
Litao Zheng: School of Life Sciences, Tsinghua University
Yan Gao: MOE Key Laboratory of Protein Science, School of Medicine, Tsinghua University
Tao Wang: MOE Key Laboratory of Protein Science, School of Medicine, Tsinghua University
Zhihui Jia: Research Centre of Microbiome, Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Capital Medical University
Haofeng Wang: Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
Yucen Huang: MOE Key Laboratory of Protein Science, School of Medicine, Tsinghua University
Mingyu Li: MOE Key Laboratory of Protein Science, School of Medicine, Tsinghua University
Quan Wang: Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology, ShanghaiTech University
Zihe Rao: MOE Key Laboratory of Protein Science, School of Medicine, Tsinghua University
Zhiyong Lou: MOE Key Laboratory of Protein Science, School of Medicine, Tsinghua University

DOI: https://doi.org/10.1038/s41467-020-19770-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 6

Abstract

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SARS-CoV-2 virus replication and transcription is mediated by the replication and transcription complex (RTC) that is composed of 16 non-structural proteins (nsp). Here, the authors present the cryo-EM structure of a SARS-CoV-2 mini RTC consisting of the viral RNA-dependent RNA polymerase with a template-primer RNA, the RdRp cofactors nsp7 and nsp8 and two nsp13 helicase molecules, and they propose a model for helicase-polymerase coupling during SARS-CoV-2 RTC assembly.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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