Expression and Correlation of Pigment Epithelium-derived Factor and Androgen Receptor in Bladder Cancer Tissues

Abstract

Objective To investigate the expression and correlation of pigment epithelium derived factor (PEDF) and androgen receptor (AR) in bladder cancer tissues. Methods The expression of PEDF and AR in 30 cases of bladder cancer tissues were detected by immunohistochemical staining. The relation between PEDF expression and AR was analyzed by Pearson correlation. We detected the expression levels of PEDF and AR in bladder cancer TCCSUP cell line and the expression of PEDF after lentivirus-mediated AR interference by Real-time PCR and Western blot. Results PEDF expression was negatively correlated with AR expression(P=0.003). Androgen could significantly upregulate AR expression and inhibit PEDF expression. At the same time, interfering AR expression could upregulate the expression of PEDF. Conclusion The expression of PEDF may be inhibited by AR expression in bladder cancer tissues.

Keywords

• bladder cancer
• pigment epithelium derived factor
• androgen receptor
• correlation