Establishment of a CRISPR/Cas9-mediated GATAD2B homozygous knockout human embryonic stem cell line

Bo Wang; Shuoji Zhu; Yuzhi Deng; Xiyalatu Sai; Zerui Chen; Jian Liu; Ge Li; Nanbo Liu; Jimei Chen; Changjiang Yu; Tucheng Sun; Ping Zhu

Stem Cell Research (Dec 2021)

Establishment of a CRISPR/Cas9-mediated GATAD2B homozygous knockout human embryonic stem cell line

Bo Wang,
Shuoji Zhu,
Yuzhi Deng,
Xiyalatu Sai,
Zerui Chen,
Jian Liu,
Ge Li,
Nanbo Liu,
Jimei Chen,
Changjiang Yu,
Tucheng Sun,
Ping Zhu

Affiliations

Bo Wang: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China
Shuoji Zhu: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China
Yuzhi Deng: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China
Xiyalatu Sai: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China; The Second School of Clinical Medicine, Southern Medical University, Guangzhou, Guangdong, China; Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao City, Inner Mongolia, Peoples Republic China
Zerui Chen: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China
Jian Liu: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China
Ge Li: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China
Nanbo Liu: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China
Jimei Chen: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China
Changjiang Yu: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China; Corresponding authors.
Tucheng Sun: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China; Corresponding authors.
Ping Zhu: Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510100, China; Corresponding authors.

Journal volume & issue: Vol. 57
p. 102590

Abstract

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As a subunit of the nucleosome remodeling and histone deacetylation (NuRD) complex, GATA zinc finger domain containing 2B (GATAD2B) plays a vital role in chromatin modification and transcriptional regulation. To further investigate the role of GATAD2B in cell fate determination of human ESCs, we generated two GATAD2B homozygous knockout human ESC lines by CRISPR/Cas9 technology. The cell line exhibits normal karyotype and typical stem cell morphology, following the high expression of pluripotent genes and differentiation potential in vitro. These cell lines will provide cell resources to investigate epigenetic regulation in extensive biological processes as menthioned above.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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