Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM)

Nadia Sarfraz; Emilia Moscoso; Therese Oertel; Harrison J. Lee; Suman Ranjit; Esther Braselmann

doi:10.1038/s41467-023-36531-y

Nature Communications (Feb 2023)

Visualizing orthogonal RNAs simultaneously in live mammalian cells by fluorescence lifetime imaging microscopy (FLIM)

Nadia Sarfraz,
Emilia Moscoso,
Therese Oertel,
Harrison J. Lee,
Suman Ranjit,
Esther Braselmann

Affiliations

Nadia Sarfraz: Department of Chemistry, Georgetown University
Emilia Moscoso: Department of Chemistry, Georgetown University
Therese Oertel: Department of Chemistry, Georgetown University
Harrison J. Lee: Department of Chemistry, Georgetown University
Suman Ranjit: Department of Biochemistry and Molecular & Cellular Biology, Georgetown University
Esther Braselmann: Department of Chemistry, Georgetown University

DOI: https://doi.org/10.1038/s41467-023-36531-y
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 9

Abstract

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No multi-color RNA fluorescent tags are currently available for use in live cells. Here, the authors show that fluorescence lifetime imaging microscopy is advantageous for multiplexed RNA visualization while achieving robust cellular contrast.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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