Single-cell quantification and dose-response of cytosolic siRNA delivery

Hampus Hedlund; Hampus Du Rietz; Johanna M. Johansson; Hanna C. Eriksson; Wahed Zedan; Linfeng Huang; Jonas Wallin; Anders Wittrup

doi:10.1038/s41467-023-36752-1

Nature Communications (Feb 2023)

Single-cell quantification and dose-response of cytosolic siRNA delivery

Hampus Hedlund,
Hampus Du Rietz,
Johanna M. Johansson,
Hanna C. Eriksson,
Wahed Zedan,
Linfeng Huang,
Jonas Wallin,
Anders Wittrup

Affiliations

Hampus Hedlund: Department of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund University
Hampus Du Rietz: Department of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund University
Johanna M. Johansson: Department of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund University
Hanna C. Eriksson: Department of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund University
Wahed Zedan: Department of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund University
Linfeng Huang: Wang-Cai Biochemistry Lab, Division of Natural and Applied Sciences, Duke Kunshan University
Jonas Wallin: Department of Mathematical Statistics, Lund University
Anders Wittrup: Department of Clinical Sciences Lund, Oncology, Faculty of Medicine, Lund University

DOI: https://doi.org/10.1038/s41467-023-36752-1
Journal volume & issue: Vol. 14, no. 1
pp. 1 – 14

Abstract

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Endosomal escape and subsequent cytosolic delivery of siRNA therapeutics is inefficient, and quantification is difficult. Here the authors report a confocal microscopy-based method to quantify cytosolic delivery of fluorescently labelled siRNA during lipid-mediated delivery.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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