Applications of real-time polymerase chain reaction for quantification of Microcystis aeraginosa(水中铜绿微囊藻定量PCR检测方法的建立)

ZHUJin-yu(朱金余); LIHai-bo(李海波); CHENFang(陈芳); CHENHong(陈红)

doi:10.3785/j.issn.1008-9497.2013.02.019

Zhejiang Daxue xuebao. Lixue ban (Mar 2013)

Applications of real-time polymerase chain reaction for quantification of Microcystis aeraginosa(水中铜绿微囊藻定量PCR检测方法的建立)

ZHUJin-yu(朱金余),
LIHai-bo(李海波),
CHENFang(陈芳),
CHENHong(陈红)

Affiliations

ZHUJin-yu(朱金余): 1. Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China( 1.浙江大学环境工程研究所，浙江　杭州　310058)
LIHai-bo(李海波): 2. Zhejiang Forestry Academy, Hangzhou 310023, China( 2.浙江省林业科学研究院，浙江　杭州　310023)
CHENFang(陈芳): 3. Hangzhou Environmental Mornitoring Central Station, Hangzhou 310007, China( 3.杭州市环境监测中心站，浙江　杭州　310007)
CHENHong(陈红): 1. Department of Environmental Engineering, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China( 1.浙江大学环境工程研究所，浙江　杭州　310058)

DOI: https://doi.org/10.3785/j.issn.1008-9497.2013.02.019
Journal volume & issue: Vol. 40, no. 2
pp. 211 – 215

Abstract

Read online

为了建立一种检测水中铜绿微囊藻浓度的定量PCR方法，分别用SDS裂解法和DNeasy Blood & Tissue Kit试剂盒提取标准品的DNA并建立标准曲线.结果表明采用DNeasy Blood & Tissue Kit试剂盒提取的标准品DNA具有很好的重复性，经定量PCR扩增得到的标准曲线R2为0.968，可以用来检测样品中铜绿微囊藻的浓度，为建立一套长期有效的测定水中铜绿微囊藻浓度的监测体系提供了技术参考.

Published in Zhejiang Daxue xuebao. Lixue ban

ISSN: 1008-9497 (Print)
Publisher: Zhejiang University Press
Country of publisher: China
LCC subjects: Science: Mathematics: Instruments and machines: Electronic computers. Computer science; Science: Physics
Website: https://www.zjujournals.com/sci/EN/1008-9497/home.shtml

About the journal

Abstract

Keywords