Screening and Optimization of Extracellular Alkaline Protease Production from Bacillus Spp

Abstract

Protease enzyme catalyzes the hydrolysis of protein. Among the various proteases, bacterial proteases are most significant when compared with animal and fungal proteases. In the present study a protease producing bacteria were isolated from soil collected from Govt. Holkar Science College, Indore campus and identified as Bacillus spp. They were grown within a temperature range between 25°C & 45 °C and pH range of 6.0 to 11.0. The optimum condition for protease production obtained was 35 °C at pH 9. The best carbon and organic nitrogen sources for this bacterial strain were fructose and yeast extract, respectively, while the most effective inorganic nitrogen sources was urea. It is envisaged that the isolate can be a potential source of alkaline protease for use as additive in industrial applications like detergent industry.

Keywords

• Alkaline protease
• Bacillus sp
• Screening