HISTOLOGICAL RESEARCH OF CANINE PANCREAS IN ACUTE DESTRUCTIVE PANCREATITIS

Abstract

The article presents the results of a histological examination of the pancreas of dogs suffering from acute destructive pancreatitis. During postmortem autopsy, histological examinations took pieces from different parts of the pancreas (at least 4 pieces from each gland). The selected pieces were fixed in a 10 % neutral aqueous formalin solution, dehydrated in ethanol of increasing concentration and poured into paraffin through chloroform. Sections 7–10 μm thick were obtained using a sled microscope. The resulting sections were stained with hematoxylin Carazzi and eosin. When conducting histological studies of the pancreas of dogs with destructive pancreatitis, we found that the microscopic changes in all the animals studied by us were similar. With destructive pancreatitis, as well as with its edematous form, pancreatic gland reveals dystrophic changes and destruction of all types of cells of pancreatic islets, which should lead to an imbalance of many enzymatic systems of the body that regulate the function of many organs. In the excretory ducts of the pancreas with destructive pancreatitis, edema of their walls, granular dystrophy, and destruction of part of the cells of these walls were detected. It was found that with destructive pancreatitis, microscopic changes in pancreatocytes were more severe than with the edematous form of the disease. In the exocrine part of the pancreas with destructive pancreatitis, edema, disorder of the acini, dystrophic changes, destruction and necrosis of pancreatocytes were recorded. The presence of necrotic changes in part of pancreatocytes in destructive pancreatitis indicates that this form of the disease can turn into necrotic pancreatitis. Thus, with destructive pancreatitis, the microscopic changes in pancreatocytes are more severe than with the edematous form of the disease. In the exocrine part of the pancreas, edema, disorganization of the acini, dystrophic changes, destruction and necrosis of pancreatocytes are recorded. The presence of necrotic changes in part of the pancreatocytosis with destructive pancreatitis indicates that this form of the disease can turn into necrotic pancreatitis. Prospects for follow-up research: subsequent histological research of dogs pancreas are planned for various forms of acute pancreatitis.

Keywords

• dogs
• pancreas
• acute destructive pancreatitis
• pancreatocytes
• histological examination