Comparative Metagenomic and Metabolomic Profiling of Rhizospheres of <i>Panax notoginseng</i> Grown under Forest and Field Conditions

Rui Shi; Haiyan Gu; Shu He; Binjie Xiong; Youguo Huang; Abraham Rami Horowitz; Xiahong He

doi:10.3390/agronomy11122488

Agronomy (Dec 2021)

Comparative Metagenomic and Metabolomic Profiling of Rhizospheres of <i>Panax notoginseng</i> Grown under Forest and Field Conditions

Rui Shi,
Haiyan Gu,
Shu He,
Binjie Xiong,
Youguo Huang,
Abraham Rami Horowitz,
Xiahong He

Affiliations

Rui Shi: Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Landscape Architecture Engineering Research Center of National Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China
Haiyan Gu: Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Landscape Architecture Engineering Research Center of National Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China
Shu He: Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Landscape Architecture Engineering Research Center of National Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China
Binjie Xiong: Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Landscape Architecture Engineering Research Center of National Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China
Youguo Huang: Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Landscape Architecture Engineering Research Center of National Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China
Abraham Rami Horowitz: Department of Entomology, Agricultural Research Organization (ARO), Gilat Research Center for Arid & Semi-Arid Agricultural Research, Rishon LeZion 85280, Israel
Xiahong He: Key Laboratory for Forest Resources Conservation and Utilization in the Southwest Mountains of China, Ministry of Education, Southwest Landscape Architecture Engineering Research Center of National Forestry and Grassland Administration, Southwest Forestry University, Kunming 650224, China

DOI: https://doi.org/10.3390/agronomy11122488
Journal volume & issue: Vol. 11, no. 12
p. 2488

Abstract

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The present study investigated the potential changes in the structure of bacterial communities and their functional profiles in the rhizospheres of Panax notoginseng cultivated under field (CK) and pine forest conditions (T). The rhizospheres of two-year-old P. notoginseng plants were used to extract DNA for metagenomic sequencing and metabolites for metabolomic profiling. The results revealed a higher root weight (p Bradyrhizobium, Candidatus koribacter and Edaphobacter, were the dominant genera in both groups. Comparatively, a higher Proteobacteria to Acidobacteria ratio was observed in forest rhizospheres than in field conditions. Candidatus Rokubacteria and Candidatus nitrostelea were identified as biomarker taxa in forest rhizospheres, while the same could be said for taxa belonging to betaproteobacteria and gammaproteobacteria, Burkholderiales and Verrucomicrobia for field rhizospheres. No differential metabolite contents were observed between the two rhizosphere groups, indicating no adverse effects of P. notoginseng cultivation on the soil quality under forest plantation.

Published in Agronomy

ISSN: 2073-4395 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Agriculture
Website: http://www.mdpi.com/journal/agronomy

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