Fine mapping and characterization of stripe rust resistance gene YrAYH in near-isogenic lines derived from a cross involving wheat landrace Anyuehong

Li Long; Jue Li; Linyu Huang; Huiling Jin; Fangnian Guan; Haipeng Zhang; Sasa Zhao; Hao Li; Zhien Pu; Wei Li; Qiantao Jiang; Yuming Wei; Jian Ma; Houyang Kang; Shoufen Dai; Pengfei Qi; Qiang Xu; Mei Deng; Youliang Zheng; Yunfeng Jiang; Matthew James Moscou; Guoyue Chen

Crop Journal (Jun 2024)

Fine mapping and characterization of stripe rust resistance gene YrAYH in near-isogenic lines derived from a cross involving wheat landrace Anyuehong

Li Long,
Jue Li,
Linyu Huang,
Huiling Jin,
Fangnian Guan,
Haipeng Zhang,
Sasa Zhao,
Hao Li,
Zhien Pu,
Wei Li,
Qiantao Jiang,
Yuming Wei,
Jian Ma,
Houyang Kang,
Shoufen Dai,
Pengfei Qi,
Qiang Xu,
Mei Deng,
Youliang Zheng,
Yunfeng Jiang,
Matthew James Moscou,
Guoyue Chen

Affiliations

Li Long: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, England, UK
Jue Li: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Linyu Huang: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Huiling Jin: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Fangnian Guan: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Haipeng Zhang: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Sasa Zhao: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Hao Li: Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Zhien Pu: College of Resources, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Wei Li: College of Resources, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Qiantao Jiang: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Yuming Wei: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Jian Ma: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Houyang Kang: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Shoufen Dai: Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Pengfei Qi: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Qiang Xu: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Mei Deng: Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Youliang Zheng: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
Yunfeng Jiang: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Corresponding authors.
Matthew James Moscou: The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, England, UK; United States Department of Agriculture-Agricultural Research Service, Cereal Disease Laboratory, St. Paul, MN 55108, USA; Corresponding authors.
Guoyue Chen: State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China; Corresponding authors.

Journal volume & issue: Vol. 12, no. 3
pp. 826 – 835

Abstract

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Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a devastating disease in wheat worldwide. Discovering and characterizing new resistance genes/QTL is crucial for wheat breeding programs. In this study, we fine-mapped and characterized a stripe rust resistance gene, YRAYH, on chromosome arm 5BL in the Chinese wheat landrace Anyuehong (AYH). Evaluations of stripe rust response to prevalent Chinese Pst races in near-isogenic lines derived from a cross of Anyuehong and Taichung 29 showed that YrAYH conferred a high level of resistance at all growth stages. Fine mapping using a large segregating population of 9748 plants, narrowed the YRAYH locus to a 3.7 Mb interval on chromosome arm 5BL that included 61 annotated genes. Transcriptome analysis of two NIL pairs identified 64 upregulated differentially expressed genes (DEGs) in the resistant NILs (NILs-R). Annotations indicated that many of these genes have roles in plant disease resistance pathways. Through a combined approach of fine-mapping and transcriptome sequencing, we identified a serine/threonine-protein kinase SRPK as a candidate gene underlying YrAYH. A unique 25 bp insertion was identified in the NILs-R compared to the NILs-S and previously published wheat genomes. An InDel marker was developed and co-segregated with YrAYH. Agronomic trait evaluation of the NILs suggested that YrAYH not only reduces the impact of stripe rust but was also associated with a gene that increases plant height and spike length.

Published in Crop Journal

ISSN: 2095-5421 (Print); 2214-5141 (Online)
Publisher: KeAi Communications Co., Ltd.
Country of publisher: China
LCC subjects: Agriculture: Agriculture (General)
Website: https://www.keaipublishing.com/en/journals/the-crop-journal/

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