Effect of Substrate Ratios and the Species of Sugar on the Antioxidant Activity of Glycosylated Products of the Rana Debris Collagen Peptide

Abstract

The active peptide (molecular weight < 3500 Da) of the enzymatic hydrolysis from Rana debris is used as a raw material of glycosylation. The peptide can be affected by environmental factors, such as pH and temperature, which can destroy structural and functional properties, so the peptide is usually modified by glycosylation. To investigate the effects of glycosylation on the antioxidant activity of Rana Debris Collagen Peptide (RDCP), glycosylated compounds were prepared using different ratios of RDCP and xylose or glucose by the Maillard reaction. The results indicated that the peptide-xylose compound (PXC) showed higher antioxidant activity than the peptide-glucose compound (PGC), and RDCP and xylose heated at a ratio of 1:4 showed good antioxidant properties. The correlation between the glycosylation degree and antioxidant activity was strong. These results indicated that glycosylation can enhance the antioxidant activity of RDCP. Such glycosylated products can be used in the field of food research.