Nature Communications (Mar 2018)
Cell-free protein synthesis from genomically recoded bacteria enables multisite incorporation of noncanonical amino acids
- Rey W. Martin,
- Benjamin J. Des Soye,
- Yong-Chan Kwon,
- Jennifer Kay,
- Roderick G. Davis,
- Paul M. Thomas,
- Natalia I. Majewska,
- Cindy X. Chen,
- Ryan D. Marcum,
- Mary Grace Weiss,
- Ashleigh E. Stoddart,
- Miriam Amiram,
- Arnaz K. Ranji Charna,
- Jaymin R. Patel,
- Farren J. Isaacs,
- Neil L. Kelleher,
- Seok Hoon Hong,
- Michael C. Jewett
Affiliations
- Rey W. Martin
- Department of Chemical and Biological Engineering, Northwestern University
- Benjamin J. Des Soye
- Chemistry of Life Processes Institute, Northwestern University
- Yong-Chan Kwon
- Department of Chemical and Biological Engineering, Northwestern University
- Jennifer Kay
- Department of Chemical and Biological Engineering, Northwestern University
- Roderick G. Davis
- Proteomics Center of Excellence, Northwestern University
- Paul M. Thomas
- Proteomics Center of Excellence, Northwestern University
- Natalia I. Majewska
- Department of Chemical and Biological Engineering, Northwestern University
- Cindy X. Chen
- Department of Chemical and Biological Engineering, Northwestern University
- Ryan D. Marcum
- Center for Synthetic Biology, Northwestern University
- Mary Grace Weiss
- Department of Chemical and Biological Engineering, Northwestern University
- Ashleigh E. Stoddart
- Department of Chemical and Biological Engineering, Northwestern University
- Miriam Amiram
- Department of Molecular Cellular, and Developmental Biology, Yale University
- Arnaz K. Ranji Charna
- Department of Chemical and Biological Engineering, Northwestern University
- Jaymin R. Patel
- Department of Molecular Cellular, and Developmental Biology, Yale University
- Farren J. Isaacs
- Department of Molecular Cellular, and Developmental Biology, Yale University
- Neil L. Kelleher
- Interdisciplinary Biological Sciences Program, Northwestern University
- Seok Hoon Hong
- Department of Chemical and Biological Engineering, Illinois Institute of Technology
- Michael C. Jewett
- Department of Chemical and Biological Engineering, Northwestern University
- DOI
- https://doi.org/10.1038/s41467-018-03469-5
- Journal volume & issue
-
Vol. 9,
no. 1
pp. 1 – 9
Abstract
Cell-free protein synthesis allows for producing proteins without the need of a host organism, thus sparing the researcher experimental hassle. Here, the authors developed a cell-free synthesis method that enables incorporating non-standard amino acids in the product.
