Nanosized titanium dioxide material. Modulation of spontaneous motility and gaba-dependent regulation of functions of stomach smooth muscles in vivo

Abstract

Electron scanning microscopy was used to obtain the image and identify the size of TiO2 nanoparticles. Using the tenzometric method in the isometric mode, it was established that chronic effect of TiO2 on stomach smooth muscles led to redistribution of the amplitudes of spontaneous contractions in terms of their frequencies. An increase in frequency of their contractions, decrease in the duration of contraction–relaxation cycle, the disturbance of the asymmetry of the duration of contraction–relaxation development, a reduction in Montevideo index of contractions and Alexandria index of contractions were demonstrated. It was also shown the existance of the divergence in numerical va­lues of frequency-amplitude complexes, TiO2-modified spontaneous contractive activi­ty of smooth muscles of stomach and caecum. In the conditions of long-term chronic influence (100 days), TiO2 removes the regulatory mechanisms of depressing a release of inhibition neuromediators from neurons of the intramural nervous interlacement, mediated by GABAA- and GABAC-receptors, in smooth musc­les of stomach and caecum.

Keywords

• stomach smooth muscle
• titanium dioxide nanoparticles
• mechanokinetics
• GABA-dependent regulation
• spontaneous and induced contractions