Culture Positivism exploitation through Automated Fluorescent-Sensor Technology from Patients with Blood Stream Infections

Abstract

This study tracks and analyses the culture results of 3615 blood samples received in Popular Diagnostic Centre, Dhanmondi, Dhaka 1205, throughout twelve month from suspected patients with blood stream infections. The samples were prepared by exploitation machine-controlled Fluorescent-Sensor Technology by BACTEC 9120® Culture System (Becton Dickinson and Company, Sparks, USA) choosing a 5 days incubation protocol. A total of 668/3615(18.50%) BACTEC 9120® system positive samples were then sub-cultured in 7% sheep blood agar, MacConkey agar, and chocolate agar plates. A sum of 346/668 (51.8%) were infective organisms and 312/668 (46.70%) positive vial cultures were contaminants. False positivism rate was 1.5% (10/668). The mean detection time for the clinical vital isolates was 17.7 h and for all the isolates was 38.1 h. Microorganisms characterization and antibiotic sensitivity testing was done using typical ways. The majorities (342, 98.84%) of those were Gram-negative microorganisms and solely 4 (1.16%) isolates were Gram-positive pathogens. Clinically vital pathogens recouped on day one, two and three were 91.0%, 7.5% and 1.5% severally. Most of the bacterial isolates were found extremely susceptible to a number of antibiotics along with ceftriaxone (95%), ceftazidine (95%), and cefepime (96%), whereas moderately sensitive to ciprofloxacin (81%), levofloxacin (89%) and chloramphenicol (81%); whereas all bacterial isolates were found to be resistant of nalidic acid (100%). Since all of our cultures were positive at intervals the primary 72 hrs, our data supported 5 day incubation system for the recuperation of medically vital microorganisms in BACTEC 9120® Culture System. [ J Adv Biotechnol Exp Ther 2020; 3(3.000): 165-170]

Keywords

• machine-controlled
• bactec 9120® system
• identification time
• antibiotic sensitivity profile