Nature Communications (Sep 2019)
Continuous artificial synthesis of glucose precursor using enzyme-immobilized microfluidic reactors
- Yujiao Zhu,
- Ziyu Huang,
- Qingming Chen,
- Qian Wu,
- Xiaowen Huang,
- Pui-Kin So,
- Liyang Shao,
- Zhongping Yao,
- Yanwei Jia,
- Zhaohui Li,
- Weixing Yu,
- Yi Yang,
- Aoqun Jian,
- Shengbo Sang,
- Wendong Zhang,
- Xuming Zhang
Affiliations
- Yujiao Zhu
- Department of Applied Physics, The Hong Kong Polytechnic University
- Ziyu Huang
- State Key Laboratory of Biobased Material and Green Papermaking; Shandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences)
- Qingming Chen
- Department of Applied Physics, The Hong Kong Polytechnic University
- Qian Wu
- State Key Laboratory for Chemical Biology and Drug Discovery, Food Safety and Technology Research Centre, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University
- Xiaowen Huang
- State Key Laboratory of Biobased Material and Green Papermaking; Shandong Provincial Key Laboratory of Microbial Engineering, Department of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences)
- Pui-Kin So
- University of Research Facility in Life Sciences, The Hong Kong Polytechnic University
- Liyang Shao
- Department of Electrical and Electronic Engineering, Southern University of Science and Technology
- Zhongping Yao
- State Key Laboratory for Chemical Biology and Drug Discovery, Food Safety and Technology Research Centre, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University
- Yanwei Jia
- State Key Laboratory of Analog and Mixed Signal VLSI, Institute of Microelectronics, University of Macau
- Zhaohui Li
- School of Electronics and Information Engineering, Sun Yat-Sen University
- Weixing Yu
- Key Laboratory of Spectral Imaging Technology, Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences
- Yi Yang
- School of Physics & Technology, Wuhan University
- Aoqun Jian
- MircoNano System Research Center, College of Information and Computer Science, Taiyuan University of Technology
- Shengbo Sang
- MircoNano System Research Center, College of Information and Computer Science, Taiyuan University of Technology
- Wendong Zhang
- MircoNano System Research Center, College of Information and Computer Science, Taiyuan University of Technology
- Xuming Zhang
- Department of Applied Physics, The Hong Kong Polytechnic University
- DOI
- https://doi.org/10.1038/s41467-019-12089-6
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 9
Abstract
Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) is a difficult enzyme to work with. Here, the authors covalently immobilized it in a microfluidic reactor to enhance its storage/thermal stabilities and reusability, which enabled the continuous artificial synthesis of glucose precursor.
