Quantitative proteomics reveals the dynamic proteome landscape of zebrafish embryos during the maternal-to-zygotic transition

Fei Fang; Daoyang Chen; Abdul Rehman Basharat; William Poulos; Qianyi Wang; Jose B. Cibelli; Xiaowen Liu; Liangliang Sun

iScience (Jun 2024)

Quantitative proteomics reveals the dynamic proteome landscape of zebrafish embryos during the maternal-to-zygotic transition

Fei Fang,
Daoyang Chen,
Abdul Rehman Basharat,
William Poulos,
Qianyi Wang,
Jose B. Cibelli,
Xiaowen Liu,
Liangliang Sun

Affiliations

Fei Fang: Department of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, MI 48824, USA
Daoyang Chen: Department of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, MI 48824, USA
Abdul Rehman Basharat: Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA
William Poulos: Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA
Qianyi Wang: Department of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, MI 48824, USA
Jose B. Cibelli: Department of Animal Science, Michigan State University, East Lansing, MI 48824, USA; Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA
Xiaowen Liu: Deming Department of Medicine, School of Medicine, Tulane University, 1441 Canal Street, New Orleans, LA 70112, USA
Liangliang Sun: Department of Chemistry, Michigan State University, 578 S Shaw Lane, East Lansing, MI 48824, USA; Corresponding author

Journal volume & issue: Vol. 27, no. 6
p. 109944

Abstract

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Summary: Maternal-to-zygotic transition (MZT) is central to early embryogenesis. However, its underlying molecular mechanisms are still not well described. Here, we revealed the expression dynamics of 5,000 proteins across four stages of zebrafish embryos during MZT, representing one of the most systematic surveys of proteome landscape of the zebrafish embryos during MZT. Nearly 700 proteins were differentially expressed and were divided into six clusters according to their expression patterns. The proteome expression profiles accurately reflect the main events that happen during the MZT, i.e., zygotic genome activation (ZGA), clearance of maternal mRNAs, and initiation of cellular differentiation and organogenesis. MZT is modulated by many proteins at multiple levels in a collaborative fashion, i.e., transcription factors, histones, histone-modifying enzymes, RNA helicases, and P-body proteins. Significant discrepancies were discovered between zebrafish proteome and transcriptome profiles during the MZT. The proteome dynamics database will be a valuable resource for bettering our understanding of MZT.

Published in iScience

ISSN: 2589-0042 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science
Website: http://www.cell.com/iscience/home

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