Cancers (Sep 2021)
Circulating Tumour Cell Numbers Correlate with Platelet Count and Circulating Lymphocyte Subsets in Men with Advanced Prostate Cancer: Data from the ExPeCT Clinical Trial (CTRIAL-IE 15-21)
- Brian Hayes,
- Lauren Brady,
- Gráinne Sheill,
- Anne-Marie Baird,
- Emer Guinan,
- Bryan Stanfill,
- Jean Dunne,
- Dean Holden,
- Tatjana Vlajnic,
- Orla Casey,
- Verena Murphy,
- John Greene,
- Emma H. Allott,
- Juliette Hussey,
- Fidelma Cahill,
- Mieke Van Hemelrijck,
- Nicola Peat,
- Lorelei A. Mucci,
- Moya Cunningham,
- Liam Grogan,
- Thomas Lynch,
- Rustom P. Manecksha,
- John McCaffrey,
- Dearbhaile M. O’Donnell,
- Orla Sheils,
- John J. O’Leary,
- Sarah Rudman,
- Ray McDermott,
- Stephen Finn
Affiliations
- Brian Hayes
- Department of Histopathology, Cork University Hospital, T12DC4A Cork, Ireland
- Lauren Brady
- Department of Histopathology and Morbid Anatomy, Trinity Translational Medicine Institute, Trinity College Dublin, D08NHY1 Dublin, Ireland
- Gráinne Sheill
- Discipline of Physiotherapy, School of Medicine, Trinity College Dublin, D08NHY1 Dublin, Ireland
- Anne-Marie Baird
- Department of Histopathology and Morbid Anatomy, Trinity Translational Medicine Institute, Trinity College Dublin, D08NHY1 Dublin, Ireland
- Emer Guinan
- Trinity Centre for Health Sciences, School of Medicine, Trinity College Dublin, D08NHY1 Dublin, Ireland
- Bryan Stanfill
- Pacific Northwest National Laboratory, Richland, WA 99352, USA
- Jean Dunne
- Department of Immunology, St James’s Hospital, D08NHY1 Dublin, Ireland
- Dean Holden
- Department of Immunology, St James’s Hospital, D08NHY1 Dublin, Ireland
- Tatjana Vlajnic
- Institute of Pathology, University Hospital Basel, 4056 Basel, Switzerland
- Orla Casey
- Cancer Trials Ireland, D11KXN4 Dublin, Ireland
- Verena Murphy
- Cancer Trials Ireland, D11KXN4 Dublin, Ireland
- John Greene
- Department of Histopathology and Morbid Anatomy, Trinity Translational Medicine Institute, Trinity College Dublin, D08NHY1 Dublin, Ireland
- Emma H. Allott
- Department of Histopathology and Morbid Anatomy, Trinity Translational Medicine Institute, Trinity College Dublin, D08NHY1 Dublin, Ireland
- Juliette Hussey
- Discipline of Physiotherapy, School of Medicine, Trinity College Dublin, D08NHY1 Dublin, Ireland
- Fidelma Cahill
- King’s College London, School of Cancer and Pharmaceutical Sciences, Translational Oncology and Urology (TOUR), London SE1 9RT, UK
- Mieke Van Hemelrijck
- King’s College London, School of Cancer and Pharmaceutical Sciences, Translational Oncology and Urology (TOUR), London SE1 9RT, UK
- Nicola Peat
- Guy’s and St Thomas’ NHS Foundation Trust, London SE1 9RT, UK
- Lorelei A. Mucci
- Department of Epidemiology, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA
- Moya Cunningham
- Department of Radiation Oncology, St Luke’s Hospital, D06HH36 Dublin, Ireland
- Liam Grogan
- Department of Oncology, Beaumont Hospital, D09V2N0 Dublin, Ireland
- Thomas Lynch
- Department of Urology, St James’s Hospital, D08NHY1 Dublin, Ireland
- Rustom P. Manecksha
- Department of Urology, St James’s Hospital, D08NHY1 Dublin, Ireland
- John McCaffrey
- Department of Oncology, Mater Misericordiae Hospital, D07AX57 Dublin, Ireland
- Dearbhaile M. O’Donnell
- HOPE Directorate, St James’s Hospital, D08NHY1 Dublin, Ireland
- Orla Sheils
- Department of Histopathology and Morbid Anatomy, Trinity Translational Medicine Institute, Trinity College Dublin, D08NHY1 Dublin, Ireland
- John J. O’Leary
- Department of Histopathology and Morbid Anatomy, Trinity Translational Medicine Institute, Trinity College Dublin, D08NHY1 Dublin, Ireland
- Sarah Rudman
- Guy’s and St Thomas’ NHS Foundation Trust, London SE1 9RT, UK
- Ray McDermott
- Department of Oncology, Tallaght University Hospital, D24NR0A Dublin, Ireland
- Stephen Finn
- Department of Histopathology and Morbid Anatomy, Trinity Translational Medicine Institute, Trinity College Dublin, D08NHY1 Dublin, Ireland
- DOI
- https://doi.org/10.3390/cancers13184690
- Journal volume & issue
-
Vol. 13,
no. 18
p. 4690
Abstract
Interactions between circulating tumour cells (CTCs) and platelets are thought to inhibit natural killer(NK)-cell-induced lysis. We attempted to correlate CTC numbers in men with advanced prostate cancer with platelet counts and circulating lymphocyte numbers. Sixty-one ExPeCT trial participants, divided into overweight/obese and normal weight groups on the basis of a BMI ≥ 25 or n = 29). CTC count positively correlated with absolute total lymphocyte count (r2 = 0.1709, p = 0.0258) and NK-cell count (r2 = 0.49, p 2 = 0.094, p = 0.0001). Correlation was also demonstrated within the overweight/obese group (n = 123, p n = 79, p = 0.001) and blood draw samples lacking platelet cloaking (n = 128, p n = 15) had a higher proportion of CD3+ T-lymphocytes (p = 0.0003) and lower proportions of B-lymphocytes (p = 0.0264) and NK-cells (p = 0.015) than the non-exercise group (n = 14). These findings suggest that CTCs engage in complex interactions with the coagulation cascade and innate immune system during intravascular transit, and they present an attractive target for directed therapy at a vulnerable stage in metastasis.
Keywords