Ganoderma lucidum mitigates gamma radiation-induced oxidative stress in Swiss albino mice

Abstract

Objective: Radiotherapy is well established in cancer therapeutics. Though radiation targets tumor cells, they damage normal tissues by bystander effect. The present study aimed to evaluate the radioprotective efficacy of Ganoderma lucidum (GL) mycelium powder on gamma radiated swiss albino mice. Materials and method: Qualitative phytochemical tests and in-vitro antioxidant activity of GL were carried out using standard protocols. Institutional animal ethical committee clearance was obtained. The pre and post-treated mice were irradiated using Gamma chamber 2000 (CARRT), Mangalore University. Biochemical estimations (TAC, SOD, NO, GPx, MDA, reduced glutathione), hematological markers (RBC, WBC, Hb, Platelet), lipid profile, inflammatory markers (Il-2, TNF-alpha, IF-gamma) and genotoxic markers (micronucleus and comet assay) were performed using standard protocols and commercially available kits. Conclusion: The pre and post-treatment groups showed statistically significant differences (p < 0.05) compared to radiation control in hematological parameters, lipid profile, micronucleus, and comet formation. In pre-treatment and post-treatment groups, Ganoderma lucidium mycelium extracts mitigated radiation-induced hematological, oxidative stress, hyperlipidemia, and genotoxic changes.

Keywords

• Radioprotection
• Antioxidants
• Ganoderma lucidium
• Hematology
• Lipid