Nature Communications (Feb 2023)
Engineering potent live attenuated coronavirus vaccines by targeted inactivation of the immune evasive viral deubiquitinase
- Sebenzile K. Myeni,
- Peter J. Bredenbeek,
- Robert C. M. Knaap,
- Tim J. Dalebout,
- Shessy Torres Morales,
- Igor A. Sidorov,
- Marissa E. Linger,
- Nadia Oreshkova,
- Sophie van Zanen-Gerhardt,
- Serge A. L. Zander,
- Luis Enjuanes,
- Isabel Sola,
- Eric J. Snijder,
- Marjolein Kikkert
Affiliations
- Sebenzile K. Myeni
- Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center
- Peter J. Bredenbeek
- Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center
- Robert C. M. Knaap
- Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center
- Tim J. Dalebout
- Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center
- Shessy Torres Morales
- Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center
- Igor A. Sidorov
- Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center
- Marissa E. Linger
- Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center
- Nadia Oreshkova
- Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center
- Sophie van Zanen-Gerhardt
- Experimental Pathology Services Lab, Central Laboratory Animal Facility, Leiden University Medical Center
- Serge A. L. Zander
- Experimental Pathology Services Lab, Central Laboratory Animal Facility, Leiden University Medical Center
- Luis Enjuanes
- Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autonoma de Madrid
- Isabel Sola
- Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autonoma de Madrid
- Eric J. Snijder
- Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center
- Marjolein Kikkert
- Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center
- DOI
- https://doi.org/10.1038/s41467-023-36754-z
- Journal volume & issue
-
Vol. 14,
no. 1
pp. 1 – 15
Abstract
In this work, authors provide a proof-of-concept study showing that deubiquitinating enzyme inactivation in MERS-CoV leads to attenuation in mice, and protection against a lethal challenge.
