Genetic Analysis for Resistance to Sclerotinia Stem Rot, Yield and Its Component Traits in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss.]

Manjeet Singh; Ram Avtar; Neeraj Kumar; Rakesh Punia; Ajay Pal; Nita Lakra; Nisha Kumari; Dalip Kumar; Anu Naruka; Mahavir Bishnoi; Rajbir Singh Khedwal; Raju Ram Choudhary; Anoop Singh; Ravindra Kumar Meena; Ankit Dhillon; Vivek K. Singh

doi:10.3390/plants11050671

Plants (Feb 2022)

Genetic Analysis for Resistance to Sclerotinia Stem Rot, Yield and Its Component Traits in Indian Mustard [<i>Brassica juncea</i> (L.) Czern & Coss.]

Manjeet Singh,
Ram Avtar,
Neeraj Kumar,
Rakesh Punia,
Ajay Pal,
Nita Lakra,
Nisha Kumari,
Dalip Kumar,
Anu Naruka,
Mahavir Bishnoi,
Rajbir Singh Khedwal,
Raju Ram Choudhary,
Anoop Singh,
Ravindra Kumar Meena,
Ankit Dhillon,
Vivek K. Singh

Affiliations

Manjeet Singh: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Ram Avtar: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Neeraj Kumar: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Rakesh Punia: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Ajay Pal: Department of Biochemistry, CCS Haryana Agricultural University, Hisar 125004, India
Nita Lakra: Department of Molecular Biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural University, Hisar 125004, India
Nisha Kumari: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Dalip Kumar: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Anu Naruka: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Mahavir Bishnoi: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Rajbir Singh Khedwal: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Raju Ram Choudhary: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Anoop Singh: Department of Botany, Maharshi Dayanand University, Rohtak 124001, India
Ravindra Kumar Meena: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Ankit Dhillon: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India
Vivek K. Singh: Department of Genetics and Plant Breeding, CCS Haryana Agricultural University, Hisar 125004, India

DOI: https://doi.org/10.3390/plants11050671
Journal volume & issue: Vol. 11, no. 5
p. 671

Abstract

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Understanding the mode of gene action that controls seed yield and Sclerotinia stem rot resistance in Indian mustard is critical for boosting yield potential. In a line × tester mating design, ten susceptible lines and four resistant testers were used to conduct genetic analysis. The significance of general combining ability (GCA) and specific combining ability (SCA) variances revealed that both additive and non-additive gene actions were involved in the inheritance of Sclerotinia stem rot resistance and yield attributing traits. In addition to 1000-seed weight and number of primary and secondary branches/plant, the genotypes RH 1569 (line) and DRMR 2035 (tester) appeared to be the strongest general combiners for Sclerotinia stem rot resistance. RH 1657 × EC 597317 was the only cross among several that demonstrated a significant desired SCA value for Sclerotinia rot resistance. Regarding SCA effects for yield and component traits, the cross RH 1658 × EC 597328 performed best, with a non-significant but acceptable negative SCA effect for resistance. DRMR 2035, RH 1222-28, RH 1569, RH 1599-41, RH 1657, RH 1658, and EC 597328 are promising genotypes to use as parents in future heterosis breeding and for obtaining populations with high yield potential and greater resistance to Sclerotinia stem rot disease in Indian mustard, based on GCA effects of parents, per se performance, and SCA effects of hybrids. Days to 50% flowering, number of primary branches/plant, main shoot length, and 1000-seed weight all had a high genotypic coefficient of variability (GCV), broad-sense heritability (h2bs), and genetic advance as percent of the mean (GAM) values, as well as significant and desirable correlations and direct effects on seed yield. As a result, these traits have been recognized as the most critical selection criterion for Indian mustard breeding programs.

Published in Plants

ISSN: 2223-7747 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Botany
Website: http://www.mdpi.com/journal/plants

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