Sterilization of different explant types in micropropagation of CAB-6p and Gisela 6 cherry rootstock

Abstract

Research with the objective of finding efficient (non residual) sterilants for the purpose of greater automatization in establishing aseptic in vitro tissue culture was conducted on cherry rootstocks CAB 6P and Gisela 6. Two ways of sterilization were examined (NaOCl and ozone O3) through survival rate on three types of explants; buds with primordial leaves, buds without primordial leaves and nodal non lignified segment with axillary buds. Ozone resulted with the high rate of survival (from 57 to 93%) regardless of the type of explants and the rootstock variety. By introducing a complete bud without destruction significantly resulted with the higher rate of survival (from 90 to 97%). Results indicate the potential for ozone sterilization of the starting material not only as an ostensible but the perforated (sub-cuticular transpiration) agent.