Pathogenicity of Salmonella enterica subspecies enterica serovar Enteritidis phage type 1 in one-day-old specific pathogen free chicks

Abstract

Background and Aim: The studies about Salmonella infection in the newly hatched chicks were not extensive. The objective of this study was to determine pathogenicity of Salmonella enterica subspecies enterica serovar Enteritidis (SE) phage type (PT) 1 in one-day-old specific pathogen free (SPF) chicks. Methods: Seventy, one-day-old SPF chicks, were divided into SE group (30 chicks), mortality group (10 chicks), both orally inoculated (1.0 mL) with SE PT1 (1x108 cfu per 1.0 mL), and one control group (30 chicks). The chicks were sacrificed at 6 and 12 hours, and days 1, 2, 3, 5, 7, 10, 14, and 21 post-inoculation (pi). Samples were collected for bacterial isolation, histological examination, and ultrastructural examination. Results: Starting from day 2 pi, the body weight in SE group significantly (p< 0.05) decreased. The SE isolation percentages from the liver, spleen, midintestinal content, caecal content, caecal tonsil, blood and cloacal swab were 0.73, 0.77, 0.33, 0.33, 0.36, 0.40 and 0.30, respectively. The isolation percentage in the liver was significantly (p [Open Vet J 2022; 12(6.000): 839-850]

Keywords

• salmonella enterica
• phage type 1
• spf chicks
• villi and crypt heights
• lesions