Prostate cancer-associated transcript 6 (PCAT6) promotes epithelial-mesenchymal transition and stemness and worsens prognosis in patients with colorectal cancer

Sun Xun; Yuan Yitao; Li Suyao; Gan Lu; Xu Midie; Li Qingguo; Liu Mengling; Hu Keshu; Nan Ke; Zhang Jiayu; Dong Yu; Lin Yufu; Zhang Xiuping; Hou Pengcong; Liu Tianshu

doi:10.3724/abbs.2024031

Acta Biochimica et Biophysica Sinica (Apr 2024)

Prostate cancer-associated transcript 6 (PCAT6) promotes epithelial-mesenchymal transition and stemness and worsens prognosis in patients with colorectal cancer

Sun Xun,
Yuan Yitao,
Li Suyao,
Gan Lu,
Xu Midie,
Li Qingguo,
Liu Mengling,
Hu Keshu,
Nan Ke,
Zhang Jiayu,
Dong Yu,
Lin Yufu,
Zhang Xiuping,
Hou Pengcong,
Liu Tianshu

Affiliations

Sun Xun: ["Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China"]
Yuan Yitao: ["Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China"]
Li Suyao: ["Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China"]
Gan Lu: ["Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China","Cancer Center, Zhongshan Hospital, Fudan University, Shanghai 200032, China","Fudan Zhangjiang Institute, Shanghai 201203, China"]
Xu Midie: ["Department of Pathology and Tissue Bank, Fudan University Shanghai Cancer Center, Shanghai, 200032, China"]
Li Qingguo: ["Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai, 200032, China","Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China"]
Liu Mengling: ["Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China"]
Hu Keshu: ["Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China"]
Nan Ke: ["Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China"]
Zhang Jiayu: ["Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China"]
Dong Yu: ["Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China"]
Lin Yufu: ["Department of Oncology, Zhongshan Hospital (Xiamen), Fudan University, Xiamen 361015, China"]
Zhang Xiuping: ["Xiamen Clinical Research Center for Cancer Therapy, Xiamen Branch, Zhongshan Hospital, Fudan University, Xiamen 361015, China"]
Hou Pengcong: ["Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China","Shanghai Institute of Precision Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200032, China"]
Liu Tianshu: ["Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China","Cancer Center, Zhongshan Hospital, Fudan University, Shanghai 200032, China"]

DOI: https://doi.org/10.3724/abbs.2024031
Journal volume & issue: Vol. 56
pp. 866 – 878

Abstract

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Approximately 20% of colorectal cancer (CRC) patients are first diagnosed with metastatic colorectal cancer (mCRC) because they develop symptoms at an advanced stage. Despite advancements in treatment, patients with metastatic disease still experience inferior survival rates. Our objective is to investigate the association between long noncoding RNAs (lncRNAs) and prognosis and to explore their role in mCRC. In this study, we find that elevated expression of PCAT6 is independently linked to unfavourable survival outcomes in The Cancer Genome Atlas (TCGA) data, and this finding is further confirmed in CRC samples obtained from Fudan University Shanghai Cancer Center. Cell lines and xenograft mouse models are used to examine the impact of PCAT6 on tumor metastasis. Knockdown of PCAT6 is observed to impede the metastatic phenotype of CRC, as evidenced by functional assays, demonstrating the suppression of epithelial-mesenchymal transition (EMT) and stemness. Our findings show the significance of PCAT6 in mCRC and its potential use as a prognostic biomarker.

Published in Acta Biochimica et Biophysica Sinica

ISSN: 1672-9145 (Print); 1745-7270 (Online)
Publisher: China Science Publishing & Media Ltd.
Country of publisher: China
LCC subjects: Science: Chemistry: Organic chemistry: Biochemistry; Science: Biology (General): Genetics
Website: https://www.sciengine.com/ABBS/home

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